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Position: Home > Articles > Research progress on single stranded DNA acquisition methods in adapter screening Acta Agriculturae Shanghai 2024,40 (2) 126-138

适配体筛选中单链DNA获取方法研究进展

作  者：
白亚龙;廖小艳;普春敏;邵毅
单  位：
关键词：
适配体;筛选;单链DNA;双链DNA;聚合酶链式反应
摘  要：
适配体技术近20年来快速发展,在食品安全、环境监测、法医鉴定、生物医药等领域都得到了高度重视.目前,已有大量研究针对小分子、蛋白质、病毒、细菌、细胞等进行了单链脱氧核糖核酸(ssDNA)适配体筛选.然而ssDNA适配体筛选仍然是一项费时费力的工作,一般都需要经过10轮左右甚至更多轮的ssDNA-dsDNA(双链DNA)-ssDNA重复步骤,直到亲和力强的序列成为主要序列,经过测序后,从中挑选一条或几条亲和力最强的序列.显然,在此过程中ssDNA的获取是适配体筛选的关键步骤,也是限速步骤.本文对适配体筛选研究中ssDNA获取的方法进行了系统综述,期望针对适配体筛选中ssDNA次级文库的获取环节可以展开更多更深入的研究,以提高筛选效率.
译  名：
Research progress on single stranded DNA acquisition methods in adapter screening
关键词：
Aptamer%Screen%Single-stranded deoxyribonucleic acid(ssDNA)%Double-stranded deoxyribonucleic acid(dsDNA)%Polymerase chain reaction(PCR)
摘  要：
Adaptor technology has developed rapidly in the past 20 years and has received high attention in fields such as food safety,environmental monitoring,forensic identification,and biomedicine.At present,a large number of studies have screened single stranded deoxyribonucleic acid(ssDNA)aptamers for small molecules,proteins,viruses,bacteria,cells,etc.However,ssDNA adapter screening is still a time-consuming and laborious task,usually requiring about 10 or more rounds of ssDNA-dsDNA(double stranded DNA)-ssDNA repeat steps until the sequence with strong affinity becomes the main sequence.After sequencing,one or several sequences with the strongest affinity are selected from them.Obviously,obtaining ssDNA during this process is a crucial step in adapter screening and also a rate limiting step.This article provides a systematic review of methods for obtaining ssDNA in adapter screening research.It is hoped that more in-depth research can be conducted on the acquisition of ssDNA secondary libraries in adapter screening to improve screening efficiency.

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