中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 籼型谷秆两用稻201高频再生系统的初步研究福建农林大学学报(自然科学版) 2002,31 (2) 146-149

Position: Home > Articles > A preliminary study on establishing high regeneration frequency system of grain-straw-dual-use-rice 201, an indica rice Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2002,31 (2) 146-149

籼型谷秆两用稻201高频再生系统的初步研究

作者：

刘峰;许明;王晓锋;郑金贵

单位：

福建农林大学生命科学学院

关键词：

谷秆两用稻;愈伤组织;分化率

摘要：

以籼型谷秆两用稻 2 0 1 (稻草粗蛋白含量比一般品种高 1倍左右 ,稻谷产量、品质与一般推广品种相当的新型水稻 )的幼胚作为试验材料 ,研究了基本培养基、琼脂质量浓度、激素、继代次数、培养基有机成分的处理方法等对其愈伤组织分化频率的影响 ,探索了谷秆两用稻高频再生体系 .此外还比较了 2 0 1与其它籼稻品种相同发育时期的幼胚在相同培养基上诱导愈伤组织的差异 .结果表明 ,NB是较合适的基本培养基 ,NB+0 .0 5 mg· L- 1 NAA +2 mg· L- 1 6-BA是较合适的分化培养基 ;适当提高分化培养基琼脂质量浓度 ,减少继代培养次数 ,对培养基有机成分进行超过滤处理等均有利于愈伤组织的分化和植株再生

译名：

A preliminary study on establishing high regeneration frequency system of grain-straw-dual-use-rice 201, an indica rice

作者：

LIU Feng, XU Ming, WANG Xiao feng, ZHENG Jin gui (College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)

关键词：

grain straw dual use rice; callus; differentiation frequency

摘要：

Grain straw dual use rice (GSDUR) 201, an indica rice, has its straw in which the crude protein content is twice as high as that in the ordinary varieties, and its grain yield and quality which are the same as those of the ordinary popularized varieties. Its young embryos were used as test materials to research the effects of treatments with basal medium, agar mass concentration, hormone, subcultures and medium organic components on its callus differentiation frequency, and to detect the high regeneration frequency system of GSDUR. In addition, the difference of callus induction on the same medium during the same growth period of young embryos of 201 and other indica rice varieties was compared. The results indicated that NB was the most suitable basal medium and NB+0.05 mg·L -1 NAA+2 mg·L -1 6 BA was the most suitable differentiation medium for the immature embryos of GSDUR, and that properly increasing the agar mass concentration of the differentiation medium, decreasing the subcultures, and ultra infiltrating the organic components of the medium were helpful to callus differentiation and explants regeneration.

相似文章

谷秆两用稻草粉代替麸皮饲喂肉猪的研究 [黄勤楼 , 郑金贵 , 陈君琛 , 林长光 , 郑开斌] 饲料与畜牧 2001 (2) 11-14
谷杆两用稻"201"引种试种初报 [李惠明] 闽东农业科技 1994,000 (2) 9-10
灿型谷秆两用稻201高频再生系统的初步研究 [刘峰, 许明] 福建农林大学学报(自然科学版) 2002,031 (2) 146-149
谷秆两和稻优质稻草栽培食用菌研究 [郑金贵, 陈群琛] 中国农业科学 2002,035 (10) 1279-1285
谷秆两用稻新品种东南201 [叶新福, 蒋家焕, 卢礼斌, 陈君琛, 黄群楼, 郑金贵] 中国农村科技 2005,000 (5) 12
谷秆两用稻202稻草养殖草鱼试验 [陈君琛, 涂杰峰, 黄勤楼, 郑金贵] 福建农业科技 2001,1 (1) 13-14
福建谷秆两用稻通过验收 [巫奕龙] 北京农业 2003,000 (5) 33
谷秆两用稻201对硒的生物富集作用研究 [陈君琛, 沈恒胜, 涂杰峰, 汤葆莎, 林文亭] 中国农学通报 2005,21 (12) 194-195+232
IPT基因遗传转化谷秆两用稻的研究 [许明, 刘峰, 郑金贵] 福建农林大学学报(自然科学版) 2003,32 (2) 205-208

导出引用

计量

文章访问数: 10

HTML全文浏览量: 0

PDF下载量: 0

籼型谷秆两用稻201高频再生系统的初步研究

相似文章

所属期刊

福建农林大学学报(自然科学版)

推荐期刊

中国农村科技

福建农林大学学报(自然科学版)