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Position: Home > Articles > THE PREPARATION AND APPLICATION OF SEVERAL ANTISERA AGAINST FISH REOVIRIS(FRV) Journal of Fisheries of China 1986,10 (4) 383-387

鱼呼肠孤病毒(FRV)抗血清的制备及其应用

作  者:
闵淑琴;洪世雯;蔡宜叔
单  位:
中国科学院武汉病毒研究所
关键词:
鱼呼肠孤病毒;草鱼出血病;抗血清
摘  要:
在FRV的分离和纯化基础上,本文介绍了制备FRV抗血清的几种方法。并采用不连续对流免疫电泳法(DCIE)和酶联免疫吸附试验(ELISA)进行FRV诊断学研究结果,同时对这两种方法检测FRV的灵敏度作了比较。
译  名:
THE PREPARATION AND APPLICATION OF SEVERAL ANTISERA AGAINST FISH REOVIRIS(FRV)
作  者:
Min Shuqin;Hong Shiwen;Cai Yiquan Wuhan Institute of Virology. Academia Sinica
关键词:
Fish reovirus;;FRV;;Hemorhage disease;;Antisera
摘  要:
A few antisera against fish reovirus(FRV) were prepared by our labor-atory. An enzyme-linked immunosorbent assay (ELISA) and discounter immunoele-ctrophoresis (DCIE)were developed to do the serologcal diagnosis of FRV infectionin grass carp, using these antisera. The DCIE was compared with ELISA for detec-tion of FRV. The ELISA was found to be at least 400 times more sensitive than theDCIE, and as a highly sensitive, rapid and specific method fof early diagnosis ofFRV.

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