Position: Home > Articles > Determination of Organic Acids in Shanxi Ageing Vinegar by High Performance Liquid Chromatography
FOOD SCIENCE
2007,28
(11)
456-459
山西老陈醋中有机酸的HPLC测定分析
作 者：
吕利华;梁丽绒;赵良启
单 位：
太原市疾病预防控制中心;山西大学化学生物学与分子工程教育部重点实验室
关键词：
离子排斥;山西老陈醋;有机酸;高效液相色谱
摘 要：
本实验建立了采用离子排斥色谱法快速测定山西老陈醋中的8种主要有机酸的方法。选用PRP-X300离子排斥色谱柱,确定最佳色谱分离条件为:0.05mmol/LH2SO4为流动相,流速1ml/min,柱温为室温,紫外检测器,检测波长为210nm,进样量5μl。各有机酸的相对标准偏差为0.72%～5.47%,回收率达到95.1%～105%。该方法具有简便快速、选择性好、准确度高的特点。
译 名：
Determination of Organic Acids in Shanxi Ageing Vinegar by High Performance Liquid Chromatography
作 者：
LU Li-hua1,LIANG Li-rong2,ZHAO Liang-qi1, (1.Chemical Biology and Molecular Engineering Laboratory of Education Ministry, Shanxi University, Taiyuan 030006, China; 2.The Center for Disease Control and Prevention of Taiyuan, Taiyuan 030001, China)
关键词：
ion-exclusion;Shanxi ageing vinegar;organic acid;HPLC
摘 要：
An ion-exclusion HPLC method for rapid determination of 8 organic acids in Shanxi ageing vinegar was developed in this paper. 8 organic acids were separated by the use of PRP-X300 ion-exclusion column, and the optimal separation conditions are as follows: 0.05mmol/L H2SO4 as mobile phase, flow rate at 1ml/min, column temperature at room temperature, UV detector at 210 nm, and sample size as 5 μl. The RSD is 0.72%～5.47%, and the recovery rate is 95.1%～105%. The method has the advantages of rapid and simple operation, good selectivity and high accuracy.
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