作 者：
王雪伟;侯名语;南云倩;王晓蕾;毕思佳;张敏;张津洛;刘立峰;崔顺立;杨鑫雷;李瑞红;冯云华;高志宝;李双路;穆国俊
单 位：
河北省秦皇岛市抚宁区农牧局;河北农业大学河北省作物种质资源实验室教育部华北作物种质资源重点实验室;河北秦皇岛市抚宁区抚宁镇农业站;河北省河间市冀宇农作物专业种植合作社
摘 要：
采用YC4和YC9组合群体,该组合在F_4代通过分子测定基因型分别为Aabb、aaBb和AaBb杂合型。通过近红外检测技术检测,YC4组合中高油酸类型的表型频率为NIR值低于80%的占19.77%,高于80%的占80.23%。YC9组合中高油酸类型的表型频率为NIR值低于80%的占28.35%,高于80%的占71.65%。考种结果表明,筛选紫色种皮高油酸花生且产量高于‘冀花5号’花生品种50%以上的材料,YC4组合群体共筛选到材料69株,中果类型50株,大果类型19株。中果类型中荚果形状多为普通型,果嘴形状多为中等或明显;大果类型中荚果形状也多为普通型,果嘴形状多为中等或明显;YC9组合群体共筛选到19株材料,中果类型13株,大果类型6株。中果类型中荚果形状多为普通型,果嘴形状多为中等或明显;大果类型中荚果形状多为普通型或茧型,果嘴形状多为中等或非常明显。通过继代选择,获得3个紫色种皮高油酸新品系ZG-4-19-5-3、ZG-4-20-18-1和ZG-9-15-9-5,油酸NIR值分别为86.92%、85.77%和88.14%,分子鉴定结果与其表型一致。该批新种质材料的创制不仅提高了高油酸花生的医疗保健价值,同时对中国高油酸花生种质资源的丰富及品质育种具有重要意义。
译 名：
Screening and Molecular Identification of High-oleic Acid New Lines with Purple Testa from Heterozygous Genotype in Peanut
作 者：
Wang Xuewei;Hou Mingyu;Nan Yunqian;Wang Xiaolei;Bi Sijia;Zhang Min;Zhang Jinluo;Liu Lifeng;Cui Shunli;Yang Xinlei;Li Ruihong;Feng Yunhua;Gao Zhibao;Li Shuanglu;Mu Guojun;North China Key Laboratory for Crop Germplasm Resources of Ministry of Education, Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei;Farming and Animal Husbandry Bureau of Funing District,Qinhuangdao City;Funing Town Agriculture Station,Funing District, Qinhuangdao City;Hejian Jiyu Agricultural Specialized Planting Cooperation;
单 位：
Wang Xuewei%Hou Mingyu%Nan Yunqian%Wang Xiaolei%Bi Sijia%Zhang Min%Zhang Jinluo%Liu Lifeng%Cui Shunli%Yang Xinlei%Li Ruihong%Feng Yunhua%Gao Zhibao%Li Shuanglu%Mu Guojun%North China Key Laboratory for Crop Germplasm Resources of Ministry of Education, Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei%Farming and Animal Husbandry Bureau of Funing District,Qinhuangdao City%Funing Town Agriculture Station,Funing District, Qinhuangdao City%Hejian Jiyu Agricultural Specialized Planting Cooperation
关键词：
Peanut;;Purple testa;;High-oleic acid;;Pod traits
摘 要：
YC4 and YC9 hybridization combinations, whose genotypes were Aabb, aaBb and AaBb identified by molecular detection in F_4, were adopted in this article. By using Near Infrared Spectrum, phenotype frequency of high-oleic acid typies in YC4 were detected. There were 19.77% individuals whose NIR value were less than 80%,and 80.23% were more than 80%. There are 28.35% individuals which NIR are less than 80% in YC9 combination and 71.65% are more than 80%. By seed investigation, the materials of high-oleic acid peanut with purple testa,of which the production was more than 50% compared with the peanut cultivar 'Jihua5', were selected in this research. In YC4 combination, a total of 69 materials were screened, with 50 plants belonging to middle pods and 19 plants to big pods. The pod traits of middle pods and big pods were mostly ordinary and the pods mouth traits were mostly medium or obvious. In YC9 combination, a total of 19 materials were screened, with 13 plants belonging to middle pods and 6 plants to big pods. The pod traits of middle pods were mostly ordinary and the pods mouth traits were mostly medium or obvious. While, the pod traits of big pods were mostly ordinary or cocoon shape and the pods mouth traits were mostly medium or very obvious. Through successive generations of selection, three purple testa with high-oleic acid lines ZG-4-19-5-3, ZG-4-20-18-1 and ZG-9-15-9-5 were obtained. The NIR values were 86.92%, 85.77%, and 88.14%, respectively, and the molecular identification results were consistent with their phenotypes. The creation of these new germplasm materials not only improves the health care value of high-oleic peanuts, but also has great significance for the enrichment of peanut germplasm resources and quality breeding in China.
