中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鹿场环境中细菌的分布及耐药性调查 中国畜牧兽医 2017,44 (3) 904-911
Position: Home > Articles > Distribution and Drug Resistance Investigation of Bacteria from Deer Farm Environment China Animal Husbandry & Veterinary Medicine 2017,44 (3) 904-911

鹿场环境中细菌的分布及耐药性调查

作  者：
王超;张梦南;乜琳;申乃文;刘鑫;张燚;何剑斌;尹荣焕
单  位：
沈阳农业大学畜牧兽医学院
关键词：
鹿场;环境;细菌分布;耐药性调查
摘  要：
为探究辽宁省西丰县鹿场环境中细菌的分布及耐药性情况,本试验采集了鹿场粪便、饮水、空气、饲料等样品,进行了细菌的分离培养及鉴定,对分离到的菌株16SrDNA基因进行PCR扩增、测序分析,并对分选出的致病菌和条件致病菌进行药敏试验。结果显示,所测样品共分离纯化出27株菌,公鹿精饲料、母鹿精饲料、粗饲料和水的含菌量分别为1.0×10~5、8.7×10~4、2.7×10~7 CFU/g和2.9×10~4 CFU/mL,表明水和粗饲料不符合国家规定的养殖场环境卫生要求;16SrDNA基因序列在NCBI比对后,鉴定出有大肠埃希菌、志贺氏菌等15种不同菌株,其中致病菌和条件致病菌共13种,这13种细菌对呋喃唑酮、多黏菌素B和头孢氨苄等耐药,对头孢曲松、环丙沙星和丁胺卡那等药物敏感。本试验结果可为鹿场细菌性疾病的防控及用药提供参考依据。
译  名：
Distribution and Drug Resistance Investigation of Bacteria from Deer Farm Environment
作  者：
WANG Chao;ZHANG Meng-nan;NIE Lin;SHEN Nai-wen;LIU Xin;ZAHNG Yi;HE Jian-bin;YIN Rong-huan;College of Animal Husbandry and Veterinary Medicine,Shenyang Agricultural University;
关键词：
deer farm;;environment;;bacterial distribution;;drug resistance investigation
摘  要：
The experiment was aimed to identify the bacterial distribution and drug resistance situation in deer farm environment form Xifeng county,Liaoning province.We collected manure,water,air and feed samples from deer farm for isolation and identification of bacteria,bacterial count,bacterial 16 SrDNA PCR amplification,sequencing analysis,and the pathogenic bacteria and conditional pathogenic bacteria of drug sensitivity test.The results showed that there were27 strains of bacteria isolated and purified,enumeration of male and female deer's pellet feed,fodder and water were 1.0×105,8.7×104,2.7×107 CFU/g and 2.9×104 CFU/mL,respectively.Description of water and fodder did not conform to the standard of the national health requirements;16SrDNA gene sequences comparison in NCBI showed that these bacteria belonged to 15 kinds of different kinds,like Escherichia coli,Shigellasp.,and so on.There were 13 kinds of pathogenic bacteria and conditional pathogenic bacteria.The 13 kinds were resistant to furazolidone,polymyxin B and cephalexin,and were sensitive to ceftriaxone,ciprofloxacin and amikacin.These results provided basis for efficient control of the disease in deer farm.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46