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当前位置: 首页 > 文章 > 保存温度对卵母细胞体外成熟率的影响及牦牛卵母细胞体外成熟培养方式初探 新疆农业科学 2012,49 (6) 1158-1164
Position: Home > Articles > Influence of Different Ovary Storage Temperature on Oocyte in Vitro Maturation and Discovery of Yak Oocyte Maturation Cultivation Methods Xinjiang Agricultural Sciences 2012,49 (6) 1158-1164

保存温度对卵母细胞体外成熟率的影响及牦牛卵母细胞体外成熟培养方式初探

作  者：
庞礴;赵新全;郭志林;屈雷;于鸿浩;郭松长
单  位：
榆林学院生命科学研究中心;中国科学院西北高原生物研究所
关键词：
牦牛;保存温度;培养方式;卵母细胞体外成熟;成熟率
摘  要：
【目的】探索不同卵巢保存温度对卵母细胞体外成熟的影响以及两种培养方式对牦牛卵母细胞体外成熟的影响。【方法】通过对绵羊、山羊、牦牛、蒙古牛和奶牛卵母细胞进行体外成熟培养,比较两种保存温度对5种卵母细胞成熟率的影响,并以牦牛卵母细胞为研究对象比较两种培养方式对牦牛卵母细胞成熟率的影响。【结果】卵巢保存温度为20～25℃时,山羊卵母细胞体外成熟率显著高于卵巢保存温度26～30℃(P=0.021)。蒙古牛卵巢保存于20～25℃时,其卵母细胞成熟率极显著高于卵巢保存在26～30℃(P=0.001)。保存温度为20～25℃时,绵羊、牦牛和奶牛的卵母细胞体外成熟率都较高于卵巢保存温度为26～30℃时的成熟率。另外,牦牛卵母细胞在四孔板中成熟培养的成熟率与在35 mm培养皿中成熟率并没有显著差异(P=0.65)。【结论】绵羊、山羊、牦牛、蒙古牛和奶牛的卵巢保存于20～25℃为宜,该卵巢保存温度可提高卵母细胞体外成熟率。牦牛卵母细胞在四孔板中培养的成熟率高于在35 mm培养皿中成熟率。
译  名：
Influence of Different Ovary Storage Temperature on Oocyte in Vitro Maturation and Discovery of Yak Oocyte Maturation Cultivation Methods
作  者：
PANG Bo1,2,3,ZHAO Xin-quan1,3,GUO Zhi-lin1,3,4,QU Lei5,YU Hong-hao5,GUO Song-chang1,3(1.Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.Key Laboratory of Adaptation and Evolution of Plateau Biota(AEPB),Northwest Institute of Plateau Biology,Chinese Academy of Sciences Xining 810001,China;4.College of Chemistry and Life Science,Qinghai University for Nationalities,Xining 810007,China;5.Life Science Research Center,Yulin University,Yulin Qinghai 719000,China)
关键词：
yak;storage temperature;cultivation method;in vitro maturation;oocyte
摘  要：
【Objective】To explore the influence of different ovary storage temperature on oocyte in vitro maturation and the influence of two cultivation methods on yak oocyte maturation.【Method】 The influence of two different ovary storage temperatures on the maturation rate of five kinds of oocytes was compared by means of in vitro maturation of sheep,goat,yak,Mongolia beef and dairy cow oocytes.Besides,two cultivation methods of yak oocyte were carried out to compare the influence on their maturation rate.【Result】As ovary storage temperature was at 20-25℃,in vitro maturation rate of goat oocyte was significantly higher than that at 26-30℃(P=0.021).When ovary was kept at 20℃ to 25℃,the Mongolia beef oocyte maturation rate was significantly higher than that at 26-30℃(P=0.001).When ovary storage temperature was at 20℃ to 25℃,the oocyte in vitro maturation rate of sheep,yak and dairy cow were higher than those at 26℃ to 30℃.In addition,the maturation rate of yak oocyte cultivated in four-well plate and 35 mm culture dish showed no significant difference(P=0.65).【Conclusion】Sheep,goat,yak,Mongolia beef and dairy cow ovary are advisable to be stored at 20℃ to 25℃,which can improve the oocyte in vitro maturation rate.The maturation rate of yak oocyte cultivated in four-well plate is higher than those cultivated in 35 mm culture dish.

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