Position: Home > Articles > INHIBITATION OF INTRODUCING ANTISENSE ACC OXIDASE GENE INTO TOMATO GENOME ON EXPRESSION OF ITS ENDOGENOUS GENE
Journal of Huazhong Agricultural University
1996
(4)
305-309
反义ACC氧化酶基因的导入对番茄乙烯生成的抑制作用
作 者：
叶志彪;李汉霞;郑用琏;刘后利
单 位：
武汉华中农业大学园艺系;武汉华中农业大学园艺系，武汉华中农业大学作物遗传改良国家重点实验室
关键词：
番茄，反义基因，基因表达，ACC氧化酶
摘 要：
将反向克隆的ＡＣＣ氧化酶基因通过Ｔｉ质粒导入到番茄基因组中，转基因番茄植株叶片和果实中ＡＣＣ氧化酶活性和乙烯产生速率均受到显著抑制，显示出反义基因能抑制靶基因（ＡＣＣ氧化酶）的表达。子代抗性标记基因的分离结果表明呈单基因遗传。
译 名：
INHIBITATION OF INTRODUCING ANTISENSE ACC OXIDASE GENE INTO TOMATO GENOME ON EXPRESSION OF ITS ENDOGENOUS GENE
作 者：
Ye Zhibiao;Li Hanxia;Zheng Yonglian;Liu Houli(Huazhong Agricultural University, Wuhan 430070)
关键词：
tomato, antisense gene, gene experession,ACC oxidase
摘 要：
The antisense cDNA clone of 1-aminocycloprapane-1-carboxylic acid (ACC)oxidase gene was ransformed into tomato genome mediated with Ti plasmid vector. The ACC oxidase activity and ethylene production rate were significantly inhibited in the leaves and fruits of transgenic plants. This shows that the expression of the endogenous ACC oxidase gene was down regulated.The inheritance of selective marker gene transferred is in the model of the simple Mendel's fashion in progenies of the self-crossed generation.
导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
