当前位置: 首页 > 文章 > 转BtCry3A基因杨树毒蛋白表达及对桑天牛抗性的研究 蚕业科学 2007,33 (4) 22-26
Position: Home > Articles > Expressions of BtCry 3A Gene in Transgenic Polar and its Resistance Against Apriona germari Science of Sericulture 2007,33 (4) 22-26

转BtCry3A基因杨树毒蛋白表达及对桑天牛抗性的研究

作  者:
甄志先;李静;梁海永;田颖川;杨敏生
单  位:
河北农业大学;中国科学院微生物研究所
关键词:
转基因杨树;BtCry3A基因;毒蛋白;桑天牛;抗虫性
摘  要:
为探索转抗虫基因(BtCry3A)741杨对鞘翅目昆虫的抗虫性,以7个转基因株系为检测对象,从分子生物学检测、毒蛋白的表达以及抗虫性检测等方面对转抗虫基因(BtCry3A)741杨株系进行了比较分析。7个转基因株系的DNA经PCR扩增显示,已转入BtCry3A基因。不同时期测定转基因株系不同部位叶片的毒蛋白含量不同,5月份测定的毒蛋白含量在当年生枝条不同部位叶片中未表现出明显的差异,至9月份封顶前,毒蛋白含量明显降低,且顶部叶片含量显著高于中下部叶片。在枝条的木质部中,毒蛋白的相对含量明显高于韧皮部。转基因株系对桑天牛幼虫的致死率很低,但对幼虫的发育具有明显的抑制作用。
译  名:
Expressions of BtCry 3A Gene in Transgenic Polar and its Resistance Against Apriona germari
作  者:
ZHEN Zhi-Xian1 LI Jing1 LIANG Hai-Yong1 TIAN Ying-Chuan2 YANG Min-Sheng1(1Agricultural University of Hebei,Baoding Hebei 071000,China;2Institute of Microbiology,The Chinese Academy of Sciences,Beijing 100080,China)
关键词:
Transgenic poplar;BtCry 3A gene;Toxin protein;Apriona germari;Resistance against insect
摘  要:
To investigate the resistance against coleopterous insects of seven clones of transgenic 741 poplar,we examed them at molecular level,and analyzed the expression of BtCry 3A gene.The results showed that Bt toxin protein content in different of clones and parts were different.There was no obvious regularity in examined leaves on twigs of 1 year old in May.In September,the content of toxins decreased obviously. The content in the upper leaves was higher than that of the lower and the middle,and that of xylem was higher than that of phloem.The clones had lower lethality to Apriona germari,but had obvious resistance against its larvae.

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