Position: Home > Articles > Hydrogen peroxide,nitro oxide and calcium participated in stomatal closure induced by elicitor PB90 from Phytophthora boehmeriae
Acta Phytopathologica Sinica
2007,37
(1)
62-68
H_2O_2、NO和Ca~(2+)参与疫病菌激发子PB90诱导烟草气孔的关闭
作 者：
张正光;王源超;蔡炳江;郑小波
单 位：
南京农业大学植保学院农业部病虫监测与治理重点开放实验室
关键词：
激发子PB90;H_2O_2;NO;Ca~(2+);气孔关闭
摘 要：
本文研究了过氧化氢(H2O2)、一氧化氮(NO)和Ca2+在棉疫病菌激发子PB90诱导烟草气孔运动中的作用。1nm o l/L激发子PB90可诱导野生型烟草Bel-W 3气孔关闭,且在相同条件下该激发子诱导反义抑制抗坏血酸过氧化物酶anti-APX烟草气孔关闭的孔径比野生型的更小;进一步药理学证明,抗氧化剂DTT和NADPH氧化酶抑制剂DPI(d ipheny-lene iodonium)、一氧化氮合酶(NO S)抑制剂L-NAM E、Ca2+螯合剂EGTA和Ca2+信号途径中CaM PKⅡ的竞争抑制剂KN93与磷脂酶C抑制剂U73122都能抵消PB90诱导气孔关闭的效应。推测该激发子PB90通过NADPH氧化酶途径形成H2O2、NO S途径形成NO和Ca2+信号途径进而诱导气孔关闭。表明H2O2、NO、Ca2+在激发子PB90诱导气孔关闭信号传递中作为第二信使起着重要的作用。
译 名：
Hydrogen peroxide,nitro oxide and calcium participated in stomatal closure induced by elicitor PB90 from Phytophthora boehmeriae
作 者：
ZHANG Zheng-guang,WANG Yuan-chao,CAI Bing-jiang,ZHENG Xiao-bo(The Key Lab of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture,College of Plant Protection,Nanjing Agric.Univ.,Nanjing 210095,China)
关键词：
elicitor PB90;hydrogen peroxide;nitro oxide;calcium;stomatal closure
摘 要：
The role of hydrogen peroxide(H2O2),nitro oxide(NO) and calcium(Ca2+) in stomatal closure of tobacco induced by elicitor PB90,a protein elicitor secreted by Phytophthora boehmeriae was investigated.Treatment to abaxial surface of young expanded leaves with 1 nmol/L PB90 could induce stomatal closure in wild-type tobacco cv.Bel-W3.Transgenic antiAPX tobacco could produce more H2O2 than Bel-W3 after treatment with PB90 and stomatal aperture of anti-APX was smaller than that of wild-type tobacco.Further experiments revealed that pretreatment with DTT,an antioxidant,DPI,an inhibitor of NADPH oxidase,L-NAME,an inhibitor of nitro oxide synthase,EGTA,a Ca2+ specific chelate,KN93,a competitively inhi-bitor of CaMPKⅡ,and U73122,an inhibitor of phospholipase C were able to attenuate PB90-triggered stomatal closure of wild-type tobacco.It was suggesting that PB90 induced stomatal closure involving H2O2 production via NADPHase,NO generation via nitro oxide synthase and Ca2+ signal transduction pathway and the molecules such as H2O2,NO and Ca2+ functioned as the second signals involved in PB90-induced stomatal closure.
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