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Position: Home > Articles > Cytotoxicity determination of several plant-derived compounds against insect cells Plant Protection 2013,39 (2) 112-116

几种植物源活性物质对昆虫细胞的毒力测定

作  者:
杨菁菁;蒋红云;张兰;沈斌斌
单  位:
中国农业科学院植物保护研究所;华南农业大学资源与环境学院
关键词:
植物源活性物质;昆虫细胞;细胞毒性
摘  要:
本文测定几种植物源活性物质对甜菜夜蛾离体培养细胞系IOZCAS-Spex-II和草地贪夜蛾离体培养细胞系Sf 9细胞增殖活性的抑制作用。试验结果表明,喜树碱、羟基喜树碱和鱼藤酮对两种昆虫细胞具有较好的抑制作用。在测定的时间(2、6、12、24、48、72h)和剂量(1.0×10-3、1.0×10-2、1.0×10-1、1.0×100,1.0×101、1.0×102μmol/L)范围内,喜树碱、羟基喜树碱和鱼藤酮对甜菜夜蛾IOZCAS-Spex-II和草地贪夜蛾Sf 9的细胞毒性具有较好的时间和剂量依赖性,而印楝素、乌头碱、次乌头碱、伪石榴皮碱、马钱子碱和吴茱萸碱对其细胞毒性具有一定的时间效应,无明显的剂量关系。
译  名:
Cytotoxicity determination of several plant-derived compounds against insect cells
作  者:
Yang Jingjing1,2,Jiang Hongyun2,Zhang Lan2,Shen Binbin1(1.College of Natural Resources and Environment,South China Agricultural University,Laboratory of Insect Ecology,Guangzhou 510642,China;2.Key Laboratory of Integrated Management of Crop Pests,Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
关键词:
plant-derived compound;insect cell;cytotoxicity
摘  要:
This study was conducted to investigate the cytotoxic effects of several plant-derived compounds on two insect cell lines,IOZCAS-Spex-II and Sf 9,established from the fatty body in the midgut of Spodoptera exigua and the embryo of S.frugiperda,respectively.The results showed that camptothecin(CPT),hydroxycamptothecin(HCPT) and rotenone had a high level of inhibitory effect on the two cells lines.The cytotoxicity of CPT,HCPT and rotenone against the two cell lines was in a time-dependent manner on time scales(2 h,6 h,12 h,24 h,48 h and 72 h) and dosage-dependent manner on dosage scales(1.0×10-3 μmol/L,1.0×10-2 μmol/L,1.0×10-1 μmol/L,1.0×100 μmol/L,1.0×101 μmol/L and 1.0×102 μmol/L).Furthermore,the other six compounds,namely azadirachtin,aconitine,hypaconitine,pseudopelletierine,strychnine and evodiamine had no significant dose-dependent cytotoxic effects,with only a time-dependent effect to some extent,on the two cell lines.

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