译 名：
Study on Detection Methods for Pollen Viability of Sinojackia sacocarpa L.Q.Luo
作 者：
Fu Qinchao;Wang Juan;Zhang Xiyu;Liu Chao;Liu Fang;Institute of Protection and Utilization of Emei Mountain Biodiversity, Leshan Normal University;
关键词：
Sinojackia sacocarpa L.Q.Luo,Pollen viability,Detection method
摘 要：
We firstly investigated the optimum condition of Sinojackia sacocarpa L.Q.Luo's pollen germination under in vitro culture condition, and then recruited four different methods to detect the pollen viability, such as triphenyltetrazolium chloride(TTC), iodine-iodide kalium(I2-KI), peroxide enzyme and acidcarmine method, in comparison with in vitro pollen germination method. Our results showed that a culture medium containing 15%sucrose and 0.01 mg/m L boric acid had the best effect on in vitro pollen germination at 23℃, and the pollen germination rate was up to 84.85%. Furthermore, we found the detection of pollen viability using peroxide enzyme, TTC, I2-KI and acidcarmine method was 78.45%, 0, 100% and 37.01%, respectively; These data suggested that peroxide enzyme method provided a quicker and more convenient means compared to other three methods, because its result was close to true viability ratio and dyeing was fast and clear. This study selected the optional condition for the pollen germination in vitro culture and identified a fast method for detecting the pollen vitality, which should provide the theoretic foundation for breeding work.
