作 者：
李钰洁;陈洪波;徐珂;何小丽;乔木;吴俊静;彭先文;梅书棋;徐忠
摘 要：
单细胞RNA测序(single cell RNA sequencing,scRNA-seq)是在单细胞水平上实现高通量测序的技术,可以用来研究特定细胞亚群基因表达的异质性,帮助我们识别未知或稀有的细胞类型,并为进一步的研究奠定基础.scRNA-seq技术的进步使其在肿瘤、临床医学和发育生物学等领域得到了广泛应用,但在畜禽育种中的研究还较少.本文介绍了scRNA-seq一般流程,着重阐述了其在畜禽的生殖分化、毛囊发育和肌肉生成等方面的研究现状以及前景,以期为scRNA-seq在畜禽遗传育种中的研究和应用提供理论依据.
译 名：
Progress in research on single cell RNA sequencing in genetics and breeding of livestock
关键词：
scRNA-seq%livestock%genetics and breeding
摘 要：
Single cell RNA sequencing(scRNA-seq)is a high-throughput sequencing technique at the single cell level,which can be used to study the heterogeneity of gene expression in specific cell subsets,help us to identify unknown or rare cell types,and lay a foundation for further research.With its progress,the scRNA-seq technology has been widely used in the fields of oncology,clinical medicine and de-velopmental biology,but it is rarely used in research on livestock and poultry breeding.This paper introduces the general process of scRNA-seq,focusing on its research status and prospect in research on reproductive differentiation,hair follicle development and muscle production of livestock and poultry,in order to provide theoretical basis for research and application of scRNA-seq in livestock and poultry genetics and breeding.
