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当前位置: 首页 > 文章 > 中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分 中国农业科学 2012,45 (7) 1387-1391
Position: Home > Articles > IL-8 Gene SNP-180A/G Affects Significantly Somatic Cell Score in Chinese Holstein Cow Scientia Agricultura Sinica 2012,45 (7) 1387-1391

中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分

作  者：
冀德君;陈仁金;杨章平;朱国强;毛永江
单  位：
扬州大学动物科学与技术学院;扬州大学畜牧兽医学院
关键词：
中国荷斯坦奶牛;IL-8基因;SCS;SNP;泌乳性状
摘  要：
【目的】通过研究IL-8基因5′侧翼区多态性与体细胞评分(somatic cell score,SCS)的相关性,获得与低体细胞计数(somatic cell counts,SCC)性状相关的遗传标记,为低SCC性状的选育提供借鉴。【方法】采用PCR-SSCP技术检测来自30个公牛家系的610头中国荷斯坦奶牛的IL-8基因5′侧翼区的多态性,并采用混合动物模型分析多态位点与主要泌乳性状及SCS的相关性。【结果】IL-8基因5′侧翼区存在1个SNP位点,即-180 A/G;定义了3种基因型,即GG、GA和AA,频率分别为0.41、0.43和0.16。该SNP位点对SCS具有显著效应,对SCS指标来说,GG极显著低于GA或AA(P<0.01),而该SNP位点对测定日产奶量、测定日乳脂率、测定日乳蛋白率和305 d校正产奶量的影响不显著(P>0.05)。【结论】IL-8基因5′侧翼区SNP-180 A/G显著影响体细胞评分。
译  名：
IL-8 Gene SNP-180A/G Affects Significantly Somatic Cell Score in Chinese Holstein Cow
作  者：
JI De-jun1,CHEN Ren-jin1,YANG Zhang-ping1,ZHU Guo-qiang2,MAO Yong-jiang1,CHEN Ying1,LI Rui1(1College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,Jiangsu;2College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,Jiangsu)
关键词：
Chinese Holstein cow;IL-8 gene;SCS;SNP;milking traits
摘  要：
【Objective】 Bovine interleukin-8(IL-8) is one of the major factors recruiting neutrophils during cow mastitis and causing rapid increase of somatic cell counts(SCC).Knowledge of IL-8 polymorphisms would be helpful to genetic selection of low SCC.【Method】 The SNPs in the 5′ upstream of bovine IL-8 gene were investigated in 610 Chinese Holstein cows from 30 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) technique,and milking traits and somatic cell score(SCS) were measured and their relationship was analyzed using a mixed animal model.【Result】 The results showed that one SNP,A(-180)G,was detected,determining three genotypes,GG,GA and AA with frequencies of 0.41,0.43 and 0.16,respectively.The SNP showed significant effect on SCS,with GG having a significantly lower SCS than GA or AA(P<0.01),while this SNP had no effect on test-day milk yield,test-day milk fat content,test-day milk protein content,and 305 d corrected milk yield.【Conclusion】The findings demonstrated that the SNP-180A/G exerted significant effect on SCS.

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