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当前位置: 首页 > 文章 > 小麦-华山新麦草易位系24-6的鉴定 麦类作物学报 2014,34 (5) 43-48
Position: Home > Articles > Identification of a Translocation Line 24-6 from Offsprings between Wheat-Psathyrostachys huashanica Journal of Triticeae Crops 2014,34 (5) 43-48

小麦-华山新麦草易位系24-6的鉴定

作  者：
苏佳妮;武军;赵继新;王秀娟;张军;贺晓岚;刘淑会;杨群慧;陈新宏
单  位：
西北农林科技大学农学院
关键词：
华山新麦草;远缘杂交;易位系;基因组原位杂交(GISH)
摘  要：
为给小麦-华山新麦草远缘杂交育种提供材料和理论基础,对普通小麦与华山新麦草杂交后代中选育出的材料24-6进行了形态学观察、细胞学检测和原位杂交(GISH)分析。结果表明:(1)通过根尖细胞学鉴定,该材料染色体数为42条,2n=42;(2)以华山新麦草Ns基因组DNA为探针,普通小麦7182基因组DNA为封阻,根尖与花粉母细胞原位杂交(GISH)显示该材料携带有2条可以配对的华山新麦草染色体臂,确定24-6为稳定的小麦-华山新麦草易位系;(3)田间观察发现24-6植株紧凑,叶色深绿,籽粒白色、呈半角质,比其母本(普通小麦7182)株高略有降低,穗长、小穗数、穗粒数、千粒重、分蘖数都有所增加。
译  名：
Identification of a Translocation Line 24-6 from Offsprings between Wheat-Psathyrostachys huashanica
作  者：
SU Jiani;WU Jun;ZHAO Jixin;WANG Xiujuan;ZHANG Jun;HE Xiaolan;LIU Shuhui;YANG Qunhui;CHEN Xinhong;College of Agronomy,Northwest A&F University;
关键词：
Psathyrostachys huashanica;;Distant hybridization;;Translocation line;;Genomic in situ hybridization(GISH)
摘  要：
Morphological observation,cytological detection and genomic in situ hybridization(GISH) analysis were carried out on a translocation line 24-6from the offsprings between Wheat-Psathyrostachys huashanicain the present paper.The results showed that:(1)This material had 42chromosomes(2n = 42)as cytology identification of root tip cells;(2)Using the genomic DNA of Psathy rostachyshuashanica Ns as a probe,and the genome DNA of common wheat 7182as blocking,GISH analysis with root tips and pollen mother cells identified two Psathyrostachys huashanica chromosomes which paired with each other in this line;(3)The field observation found that the line 24-6had dark green leaf color,white grain(half horniness),slightly shorter plant height than the female parent.The spike length,spikelet number,grain number per spike,and tiller number were all increased.Consequentially,24-6is a stable wheat-Psathyrostachys huashanica translocation line, which could be exploited as an intermediate material in wheat genetic and breeding programs.

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