单 位：
浙江省农产品质量监督检验测试中心;浙江工业大学药学院
关键词：
五谷虫;脂肪酸;抗肿瘤;抗HIV-1整合酶
摘 要：
为研究特种五谷虫(大头金蝇Chrysomya megacephala蝇蛆的俗称)脂肪酸的体外抗肿瘤、抗HIV-1整合酶活性,并确定其组分构成,通过溶剂萃取和油脂酶解获得2种脂肪酸FA1和FA2,采用MTT法/SRB法测定其体外抗人白血病细胞HL-60/人肺癌细胞A-549活性,以及采用Biotin-ELISA法检测其对HIV-1整合酶的抑制作用;并由GC-MS分析确定其脂肪酸组成。结果表明:FA1和FA2对人白血病细胞/人肺癌细胞均有显著的抑制活性,其IC50值在35~65μg/mL;对HIV-1整合酶同样具有强烈的抑制活性,IC50值分别为86.7μg/mL和98.5μg/mL。GC-MS分析表明,FA1和FA2化学组成相似,均含有15%~16%的多不饱和脂肪酸(PUFA),其中有2个组分为ω-6PUFA。提示特定培养的五谷虫,其脂肪酸成分具有显著的体外抗肿瘤、抗HIV-1整合酶活性,其中含有的PUFA,尤其ω-6PUFA,可能是主要活性组分;FA2在来源上和FA1存有相关性。
译 名：
Inhibitory effect of fatty acids from specifically-cultivated Chrysomya megacephala (Fabricius) (Diptera:Calliphoridae) larvae on tumor cells and HIV-1 integrase in vitro and their ingredient analysis
作 者：
HUA Yun-Fen1,WU Jiang-Lin2,QIAN Jun-Qing1(1.College of Pharmaceutical Sciences,Zhejiang University of Technology,Hangzhou 310014,China;2.Center of Inspection and Testing for Quality of Agriculture Products,Zhejiang Province,Hangzhou 310020,China)
关键词：
Chrysomya megacephala larvae;fatty acid;anti-tumor;anti-HIV-1 integrase
摘 要：
The fatty acids FA1 and FA2 from Chrysomya megacephala(Fabricius)larvae specifically-cultivated with a patented method were obtained by solvent extraction and oil enzymolysis.Their inhibitory effect on tumor cells in vitro was studied by MTT assay and SRB assay,as the antagonistic effect on HIV-1 integrase was studied by Biotin-ELISA.The ingredient analysis of the fatty acids was carried out by GC-MS.The results showed that both FA1 and FA2 had remarkable inhibitory activities against human leukemia cells HL-60 and human lung cancer cells A-549,with IC_50 ranged within 35-65 μg/mL.And both of them were also found to inhibit HIV-1 integrase intensively,with IC_50 86.7 μg/mL and 98.5 μg/mL,respectively.The results of GC-MS analysis showed the similarity of chemical composition between FA1 and FA2,with the same content of 15%-16% PUFA,from which two ω-6 PUFAs were identified.Thus it is concluded that the fatty acids from specifically-cultivated C.megacephala larvae have significant inhibitory effect on tumor cells and HIV-1 integrase in vitro,with PUFA,especially ω-6 PUFA,as the main active components potentially.In addition,FA2 was inferred to have correlation with FA1 in origin.
