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当前位置: 首页 > 文章 > 切花月季无性系~(60)Co γ射线离体诱变研究 核农学报 2009,23 (2) 71-75
Position: Home > Articles > MUTAGENESIS OF THE SOMACLONES in vitro OF CUT ROSES BY ~(60)Co γ-RAYS IRRADIATION Journal of Nuclear Agricultural Sciences 2009,23 (2) 71-75

切花月季无性系~(60)Co γ射线离体诱变研究

作  者：
瞿素萍;唐开学;王继华;苏艳;王丽花;张颢
单  位：
云南省农业科学院花卉研究所;云南省农业科学院质量标准与检测技术研究所;农业部花卉产品质量监督检验测试中心
关键词：
月季;无性系;~(60)Coγ射线;辐照诱变
摘  要：
以月季叶片愈伤组织和再生的不定芽2种材料为外植体,对其体细胞无性系进行离体辐射诱变,研究了~(60)Coγ辐照对叶片愈伤组织再生率、不定芽增殖率及后期生长情况的影响。结果表明,~(60)Coγ射线处理大大降低了叶片愈伤组织的再生频率,叶片再生不定芽更适宜作为离体辐照诱变材料。不定芽的适宜诱变剂量为50～60Gy,由回归方程导出其致死剂量为122Gy,半致死剂量约为76Gy。
译  名：
MUTAGENESIS OF THE SOMACLONES in vitro OF CUT ROSES BY ~(60)Co γ-RAYS IRRADIATION
作  者：
QU Su-ping 1,2 TANG Kai-xue1 WANG Ji-hua 1,3 SU Yan 1,2 WANG Li-hua 1,2 ZHANG Hao 3(1.Quality Standard and Testing Technology Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205;2.Supervision and Testing Centre for Flowers of Ministry of Agriculture,Kunming,Yunnan 650205;3.Flower Research Institute of Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205)
关键词：
rose;somaclones;60 Co γ-rays;radiation mutation;
摘  要：
Mutagenesis of cut rose in vitro irradiated by 60 Co γ-rays was studied.The callus of leaves and regenerations adventitious bud was used as the explants for mutagenesis.Effect of 60 Co γ-rays irradiation on the callus's regeneration rate,adventitious bud's multiplication rate and vegetal status were studied.The results showed that the regeneration frequency of callus was decreased by 60 Co γ-rays irradiation.The regenerations adventitious bud was the better experimental materials compared with the callus of leaves.The lethal dose was 122Gy and the semi-lethal dose was 76Gy according to the regression equation.The appropriate dose on adventitious bud by irradiation rays was 50～60Gy.

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