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当前位置: 首页 > 文章 > 猪伪狂犬病病毒山西株胸苷激酶基因序列分析 中国畜牧兽医 2014,41 (4) 12-17
Position: Home > Articles > Sequence Analysis of TK Gene of Porcine Pseudorabies Virus Isolated from Shanxi Area China Animal Husbandry & Veterinary Medicine 2014,41 (4) 12-17

猪伪狂犬病病毒山西株胸苷激酶基因序列分析

作  者：
樊振华;孟帆;吴忻;刘文俊;姚敬明;王娟萍;韩一超;米瑞娟;雷宇平
单  位：
山西省动物疫病预防控制中心;山西省农业科学院畜牧兽医研究所
关键词：
猪伪狂犬病病毒;TK基因;变异分析
摘  要：
采用PCR方法对2011—2013年山西省分离的2株猪伪狂犬病病毒(pseudorabies virus,PRV)的胸苷激酶(TK)基因进行了克隆和测序,并将其基因序列和推导的氨基酸序列与国内外主要流行毒株Bartha株、Becker株、Ea株、Kaplan株、LA株、Min-A株、NIA-3株、SH株、SL株和Yangsan株进行了同源性分析。序列分析结果表明,核苷酸同源性分别为99.7%、99.6%、99.8%、99.7%、99.7%、94.4%、99.1%、57.2%、99.5%、99.7%;氨基酸同源性分别为99.1%、99.1%、99.7%、99.4%、99.4%、90.3%、98.1%、49.7%、98.8%、99.1%。另外在核苷酸序列中起始密码子上游有3段GC box的序列,终止密码子下游有多聚腺苷加尾信号AATAAA;TK基因均由320个氨基酸组成,氨基酸序列中有疱疹病毒TK基因共同的保守序列-R*Y*DG**G*GK*T-和-FDRHP*A***C*P*AR-。
译  名：
Sequence Analysis of TK Gene of Porcine Pseudorabies Virus Isolated from Shanxi Area
作  者：
FAN Zhen-hua;MENG Fan;WU Xin;LIU Wen-jun;YAO Jing-ming;WANG Juan-ping;HAN Yi-chao;MI Rui-juan;LEI Yu-ping;Institute of Animal Husbandry and Veterinary Medicine,Shanxi Academy of Agricultural Sciences;Shanxi Veterinary Prevention and Treatment Station;
关键词：
porcine pseudorabies virus;;TKgene;;variation analysis
摘  要：
TKgenes of two pseudorabies virus(PRV)strains that had been isolated from Shanxi province were amplified by RT-PCR among 2009and 2011,cloned and sequenced.The obtained sequences and the deduced amino acid were analyzed and compared with the domestic and international major epidemic strains of PRV Bartha strain,Becker strain,Ea strain,Kaplan strain,LA strain,Min-A strain,NIA-3strain,SH strain,SL strain and Yangsan strain by homologous analysis.Sequence analysis showed that the nucleotide homologies of TKgenes were 99.7%,99.6%,99.8%,99.7%,99.7%,94.4%,99.1%, 57.2%,99.5%and 99.7%,respectively,and the amino acid homologies were 99.1%,99.1%,99.7%,99.4%,99.4%, 90.3%,98.1%,49.7%,98.8%and 99.1%.3sections of GC box like sequence upstream of the initiation codon and poly adenosine polyadenylation signal AATAAA downstream of the stop codon were found in nucleotide sequence,TK gene was composed of 320amino acids,with a conserved sequence of TKgene of herpesvirus common amino acid sequence,namely-R* Y*DG**G*GK*T-and-FDRHP*A***C*P*AR-.

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