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当前位置: 首页 > 文章 > 蛔虫抗原基因ALAg克隆及序列分析 黑龙江畜牧兽医 2011 (17) 13-15
Position: Home > Articles > Cloning and sequence analysis of ALAg antigen gene from Ascaris lumbricoides Heilongjiang Animal Science and Veterinary Medicine 2011 (17) 13-15

蛔虫抗原基因ALAg克隆及序列分析

作  者：
何光志;田维毅;王平;王文佳;奚锦
单  位：
贵阳中医学院凤冈县天桥乡畜牧兽医水产站兴义市乌沙镇格里小学兴义市捧乍中学;贵阳中医学院基础部;贵阳中医学院
关键词：
蛔虫;抗原基因ALAg;克隆及序列分析
摘  要：
为了确定蛔虫基因工程疫苗的候选基因,试验以蛔虫幼虫的RNA为模板,利用RT-PCR方法扩增出ALAg基因,扩增产物克隆到pMD18-T载体,筛选阳性克隆测序并进行Blast分析。结果表明:该基因与GenBank公布的猪蛔虫As37抗原基因(AB078971)的同源性为95%,与西式贝蛔虫Ag3抗原基因(EU927450)的同源性为92%。说明ALAg基因是线虫特有的抗原基因。
译  名：
Cloning and sequence analysis of ALAg antigen gene from Ascaris lumbricoides
作  者：
HE Guang-zhi1,TIAN Wei-yi1,WANG Ping1,WANG Wen-jia1,XI Jin1,YU Qi1, CAO Feng1,HUANG Gao1,CAI Kun1,HAN Jie1,Wang Qian-yu1,LIU An-sheng2, AN Chuan-wei1,CHA Gao-wu3,ZHANG Peng4(1.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China;2.Feng gang County Tianqiao Township Animal Husbandry and Veterinary Station,Fenggang 564217,China;3.Xingyi Geli Primary School,Guizhou Wusha Town,Xingyi 562405,China;4.Xingyi Pengzha Middle School,Xingyi 562407,China)
关键词：
Ascaris lumbricoides;ALAg;cloning and sequence analysis
摘  要：
To determine the candidate genes of gene engineering vaccine for Ascaris lumbricoides,this experiment selected the RNA as a template from larva of Ascaris lumbricoides.The ALAg gene from Ascaris lumbricoides was amplified by RT-PCR.The amplification product was cloned into pMD18-T vector,then the positive clones were sequenced and analyzed.A search of the public databases revealed that ALAg shared 95% similarity with the nucleotide sequence of the As37(AB078971) antigen gene from Ascaris suum,and 92% similarity with the nucleotide sequence of Ag3 from Baylisascaris schroederi(EU927450).It suggests that the ALAg predicted by the cDNA sequence is a nematode-specific antigen gene.

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