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当前位置: 首页 > 文章 > 养殖废水中异养硝化细菌的分离筛选和鉴定 农业环境科学学报 2010,29 (11) 2232-2237
Position: Home > Articles > Isolation and Identification of Heterotrophic Nitrifiers from Cultivation Wastewater Journal of Agro-Environment Science 2010,29 (11) 2232-2237

养殖废水中异养硝化细菌的分离筛选和鉴定

作  者：
刘芳芳;周德平;吴淑杭;张明;褚长彬;范洁群;姜震方
单  位：
上海市农业科学院生态环境保护研究所;华东师范大学环境科学系
关键词：
养猪场废水;异养硝化细菌;分离;脱氮能力;粪产碱杆菌
摘  要：
为了获得脱氮功能强的异养硝化菌株用于养殖废水的脱氮处理,通过富集、分离和纯化等步骤,并结合格利斯试剂检验菌株硝化能力的方法,从某养猪场污水处理池污泥中分离筛选了4株具异养硝化功能的菌株,分别标号为79、84、L116、L117,通过16SrDNA序列分析和美国全自动微生物分析仪Biolog鉴定,4株菌均为粪产碱杆菌(Alcaligenesfaecalis),并验证了这4株菌的硝化能力。结果表明,当液体培养基初始氨氮浓度为90mg.L-1左右时,在振荡培养48h内,菌株79、84、L116、L117培养基中氨氮和总氮均快速下降,氨氮去除率分别达到44.4%、47.9%、61.3%和56.4%,总氮(除菌)去除率达到39.9%、38.5%、43.4%和40.7%。
译  名：
Isolation and Identification of Heterotrophic Nitrifiers from Cultivation Wastewater
作  者：
LIU Fang-fang1,2,ZHOU De-ping2,WU Shu-hang1,2,ZHANG Ming1,CHU Chang-bin2,FAN Jie-qun2,JIANG Zhen-fang2(1.Department of Environment Science,East China Normal University,Shanghai 200063,China;2.Institute of Eco-Environmental Protection,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China)
关键词：
piggery wastewater;heterotrophic nitrifier;isolation;denitrification capacity;Alcaligenes faecalis
摘  要：
The biological nitrogen removal is widely applied in the wastewater treatment,and it is more economic in all nitrogen removal methods.The traditional biological nitrogen removal method is mainly dedicated to the research of autotrophic nitrobacteria.Compared to the autotrophic nitrobacteria,heterotrophic nitrification bacteria are able to utilize organic carbon sources and inorganic carbon sources.What's more,they have absolute superiority in adaptive capacity and can widely exist in the environment.In order to get the heterotrophic nitrification bacteria which can remove nitrogen in cultivation wastewater,four strains of heterotrophic nitrifiers from the sewage of an hoggery in Congming(Shanghai)through the procedure of enriching,separating,purifying and identificating their nitrification capacity by the principle of when nitrite meets Eglise reagent,it will become red.The four strains were given the labels of 79,84,L116 and L117.The 16S rDNA sequence analysis and the United States automatic microbial analyzer Biolog were used to identify the strains.The result showed that the four strains were Alcaligenes faecalis.The result of experiment showed that when the initial ammonia-nitrigon concentration reached 90 mg.L-1,the nitrogen removal rates of 79,84,L116 and L117 within 48 h were 44.4%,47.9%,61.3% and 56.4% espectively and the removal rates of total nitrogen were 39.9%,38.5%,43.4% and 40.7%,respectively.Furthermore,there exisited nitrite in the process of denitrification.

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