Position: Home > Articles > Cloning and function analysis of CiF3H gene
Journal of Northeast Agricultural University
2019
(11)
53-60
CiF3H基因克隆及功能分析
作 者：
杨飞芸;白洁;杨天瑞;王瑞刚
单 位：
内蒙古农业大学生命科学学院内蒙古自治区植物逆境生理与分子生物学重点实验室;内蒙古农业大学食品科学与工程学院
关键词：
黄烷酮-3-羟化酶(F3H);中间锦鸡儿;总黄酮含量;花青素含量;转基因拟南芥
摘 要：
黄烷酮-3-羟化酶(F3H)是类黄酮合成途径的中枢位点,调控代谢途径中黄酮醇和花青素合成。文章根据转录组数据库中F3H基因中间片段设计特异引物,以中间锦鸡儿cDNA为模板,克隆得到CiF3H基因全长,通过序列分析、系统进化分析加以确认。qRT-PCR检测分析发现,中间锦鸡儿CiF3H基因受紫外、NaCl和ABA等胁迫诱导时表达模式具有相同规律。CiF3H基因在叶和根中表达量相当,茎中较少。异源表达CiF3H基因拟南芥的总黄酮含量少量增加,花青素含量降低。
译 名：
Cloning and function analysis of CiF3H gene
作 者：
YANG Feiyun;BAI Jie;YANG Tianrui;WANG Ruigang;School of Life Sciences, Inner Mongolia Agricultural University;Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology;School of Food Science and Engineering, Inner Mongolia Agricultural University;
关键词：
Flavanone-3-hydroxyase(F3H);;Caragana intermedia;;total flavonoids content;;anthocyanins content;;transgenic Arabidopsis
摘 要：
Flavanone-3-hydroxyase(F3 H) is the essential site in flavonoids biosynthesis pathway,which regulates the synthesis of flavonols and anthocyanins in the whole metabolic pathway. In this study, specific primers were designed according to the intermediate fragment of F3 H gene in the transcriptome database, and the full length of CiF3 H gene sequence was cloned using Caragana intermedia cDNA as template, which was confirmed by sequence and phylogenetic analyses. The expression of CiF3 H had the same pattern by ultraviolet(UV), NaCl and abscisic acid(ABA) stresses as revealed by quantitative real-time PCR(qRT-PCR). The expression of CiF3 H was similar in leaves and roots, but less in stems. The content of total flavonoids in transgenic Arabidopsis increased slightly, but the content of anthocyanins decreased.
相似文章
-
三色堇花色素合成途径中部分结构基因的克隆及生物信息学分析 [龚胜, 孙海燕, 张珂, 曾媛, 李婧, 王健, 李新国] 西北农林科技大学学报(自然科学版) 2015,43 (7) 133-142
