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当前位置: 首页 > 文章 > 马立克氏病淋巴瘤的细胞化学及图像分析 中国兽医学报 1991 (4) 335-339
Position: Home > Articles > Cytochemistry and Image Analysis of Marek's Disease Lymphe- ma Chinese Journal of Veterinary Science 1991 (4) 335-339

马立克氏病淋巴瘤的细胞化学及图像分析

作  者：
薄清如;郑兆荣;邱震东;刘宝岩;高丰;程相朝;黄建珍
单  位：
兽医大学病理教研室
关键词：
MD;淋巴瘤细胞;ATPase;ACP;ANAE;核面积;核积分光密度;核平均光密度
摘  要：
经ATPase、ACP和ANAE染色表明,马立克氏病(MD)淋巴瘤中具有T细胞标志的约占66%,B细胞标志的约占25%,巨噬细胞约占9%.MD肿瘤按细胞形态分为两类:单一小淋巴细胞肿瘤和多形态的淋巴细胞肿瘤.图像分析表明,前者肿瘤细胞比较小,大小均匀一致,核平均面积3.24±0.59μm~2,仅为正常胸腺细胞核面积的一半,其中2～4μm~2的细胞占90%以上,分布集中.后者肿瘤细胞形态各异,大小不一,同一病鸡不同脏器的肿瘤,在细胞核平均面积、核积分光密度、核平均光密度方面差异不显著,从而证实MD内脏肿瘤细胞的一致性.
译  名：
Cytochemistry and Image Analysis of Marek's Disease Lymphe- ma
作  者：
Bo Qingru et al (Department of Pathology, Veterinary College of PLA)
关键词：
MD; lymphoma cells; ATPase; ACP; ANAE staining; nucleus area; light to-tal density of nucleus; light mean density of nucleus
摘  要：
In the Marek's disease (MD) tumor-tissue, there are 66% T-marker cells, 25% B-marker cells and 9% macrophages as indicated by ATPase, ACP and ANAE staining. According to the cellular morphology ,the tumor could be classified into two kinds: one contains small-siged lymphocytes, and the other consists of polymorphic ones. Image analysis showed that the cells of the former one are uniform in size, the average nucleus area is 3. 25±0. 592μm2 (mostly 2- 4μm2) , merely a half of that of the normal thymocytes, they distribute intensively, wheras in the latter one the cells reveal various morphologic features and are different in size. There are no statistical differences in average area of nucleus, light total density and light mean density of nucleus in various affected organs in the same sicked chicken, which thus confirmed that the visceral tumor cells Marek's disease are unanimous.

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