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当前位置: 首页 > 文章 > 液相色谱-串联质谱法同时测定养殖水体中孔雀石绿和结晶紫含量 中国渔业质量与标准 2018 (1) 49-54
Position: Home > Articles > Simultaneous determination of malachite green and crystal violet level in aquaculture water using HPLC-MS/MS Chinese Fishery Quality and Standards 2018 (1) 49-54

液相色谱-串联质谱法同时测定养殖水体中孔雀石绿和结晶紫含量

作  者：
丁勇;张秀妍;王骏;马琳
单  位：
大连市水产技术推广总站
关键词：
孔雀石绿;结晶紫;养殖水体;液相色谱-串联质谱(LC-MS/MS);内标法
摘  要：
本研究建立了应用液相色谱-串联质谱(LC-MS/MS)同时测定养殖水体中孔雀石绿和结晶紫的含量的方法。水样经乙腈和二氯甲烷萃取浓缩,中性氧化铝小柱净化,1 m L 50%的乙腈-乙酸铵溶液定容,氘代孔雀石绿和氘代结晶紫内标法定量,液相色谱-三重四级杆串联质谱仪多反应监测扫描模式(MRM)检测养殖水体中孔雀石绿和结晶紫的含量。结果表明,方法的定量限为2.0 ng/L;标准曲线线性范围为0.2~20.0 ng/m L;加标水平在2.0~20.0 ng/L之间时,平均回收率为89.9%~102.0%,批内和批间相对标准偏差均小于10%。该方法稳定可靠,可适用于检测养殖水体中孔雀石绿和结晶紫的含量。
译  名：
Simultaneous determination of malachite green and crystal violet level in aquaculture water using HPLC-MS/MS
作  者：
DING Yong;ZHANG Xiuyan;WANG Jun;MA Lin;Dalian Fisheries Technical Extension Station;
关键词：
malachite green;;crystal violet;;aquaculture water;;liquid chromatography-tandem mass spectrometry
摘  要：
In this study,a high performance liquid chromatography-tandem mass spectrometric( HPLC-MS/MS)method has been adopted to determine stimultaneously malachite green and crystal violet in aquaculture water. Samples were extracted by acetonitrile and dichloromethane,neutral alumina column purification and constant volume with1 m L acetonitrile ammonium acetate solution prior to HPLC-MS/MS analysis. The analysis was carried out with the multi-reaction monitoring( MRM) mode and internal standard isotope dilution method was used for quantification.Calibration curves for malachite green and crystal violet exhibited good linearity over the concentration range from 0. 2 to 20. 0 ng/m L with quantification limits of 2. 0 ng/L. When the spiked level was 2. 0 to 20. 0 ng/L,average recovery rate was between 89. 9% and 102. 0%. Intraplate relative standard deviation was less than 10%,and inter-batch relative standard deviation was less than 10%. This indicated that the method was accurate and reliable to meet the requirements of detection for malachite green and crystal violet in aquaculture water. [Chinese Fishery Quality and Standards,2018,8( 1) : 49-54]

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