中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing 水稻科学(英文版) 2006,13 (4) 234-242

Position: Home > Articles > Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing Rice Science 2006,13 (4) 234-242

Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing

作者：

Yi Zi-li;Wang Zi-xuan;Qin Jing-ping;Jiang Jian-xiong;Tan Yan-ning;Zhou Qing-ming

单位：

Changsha 410128; China; Hunan Agricultural University;Key Laboratory of Cell Engineering

关键词：

hybrid rice;lysozyme gene;rice blast;backcrossing;breeding

摘要：

A lysozyme gene resistant to rice blast was transferred from the donor transgenic japonica rice Zhonghua 9 (D2-1 -2) into a sterile line Pei'ai 64S(PA 64S) and restorer line 9311 of the two-line hybrid rice Liangyoupeijiu, and the restorer line Minghui 63 (MH 63) of three-line hybrid rice Shanyou 63 by successive backcrossing. The PCR analysis confirmed that foreign lysozyme gene was segregated at ratio of 1:1 in backcross generations of B_39311, B_3MH63 and B_2PA64S, and at ratio of 3:1 in selfed generations of B_2F_2 9311, B_2F_2 MH63 and B_1F_2 PA64S, indicating that the foreign gene was stably inherited over successive generations as a dominant single copy gene. The resistance against rice blast in backcross or selfed generations and corresponding testcross combinations were investigated in 2003 and 2004. The results showed that the resistance of the transgenic rice to blast had a greater improvement than that of the corresponding recurrent parents or the corresponding check hybrid combinations. The resistance of the advanced backcross and selfed generations to rice blast is much stronger than that of the early generations. The study confirmed that transferring the lysozyme gene into hybrid parents by backcrossing was a simple and effective approach to develop new hybrid rice resistant to rice blast.

相似文章

Grain Quality and Genetic Analysis of Hybrids Derived from Different Ecological Types in Japonica Rice (Oryza sativa) [LENG Yan, HONG De-lin] 水稻科学(英文版) 2004,11 (4) 165-170
Combined Effects of Nutrient and Pesticide Management on Soil Microbial Activity in Hybrid Rice Double Annual Cropping System [XIE Xiao-mei, LIAO Min, LIU Wei-ping, Susanne KLOSE] 水稻科学(英文版) 2004,11 (3) 140-146
Effect of System of Rice Intensification on Grain Plumpness in Association with Source-Sink Ratio in Mid-Season Hybrid Rice [Xu Fu-xian, XIONG Hong, ZHU Yong-chuan, WANG Gui-xiong] 水稻科学(英文版) 2004,11 (5/6) 269-273
Effect of Temperature on Pollen Fertility in Inter-Subspecific Rice Hybrids [YANG Jie, WAN Jian-min, ZHAI Hu-qu, WANG Cai-lin, ZHONG Wei-gong, ZOU Jiang-shi] 水稻科学(英文版) 2004,11 (4) 195-199
Genetic Diversity among Parents of Hybrid Rice Based on Cluster Analysis of Morphological Traits and Simple Sequence Repeat Markers [WANG Sheng-jun, LU Zuo-mei, WAN Jian-min] 水稻科学(英文版) 2006,13 (3) 155-160
Breeding Rice Restorer Lines with High Resistance to Bacterial Blight by Using Molecular Marker-Assisted Selection [Deng Qi-ming, Wang Shi-quan, Zheng Ai-ping, Zhang Hong-yu, Li Ping] 水稻科学(英文版) 2006,13 (1) 22-28
Source-Sink and Grain-Filling Characteristics of Two-Line Hybrid Rice Yangliangyou 6 [Zhao Bu-hong, Wang Peng, Zhang Hong-xi, Zhu Qing-sen, YANG Jian-chang] 水稻科学(英文版) 2006,13 (1) 34-42
Response to High Temperature in Flag Leaves of Super High-Yielding Rice Pei'ai 64S/E32 and Liangyoupeijiu [PENG Chang-lian, Ou Zhi-ying, LIU Nan, LIN Gui-zhu] 水稻科学(英文版) 2005,12 (3) 179-186
Effect of Rolled Leaf Gene Rl_((t)) on Grain Quality in Hybrid Rice [SHAO Yuan-jian, CHEN Zong-xiang, CHEN Hong-qi, ZHANG Ya-fang, PAN Xue-biao] 水稻科学(英文版) 2005,12 (3) 168-172
Forty Years' Development of Hybrid Rice: China's Experience [CHENG Shi-hua, CAO Li-yong, YANG Shi-hua, ZHAI Hu-qu] 水稻科学(英文版) 2004,11 (5/6) 225-230

导出引用

计量

文章访问数: 5

HTML全文浏览量: 0

PDF下载量: 0

Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing

相似文章

所属期刊

水稻科学(英文版)

推荐期刊

农业科学学报 (英文)

水稻科学(英文版)