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当前位置: 首页 > 文章 > 基于短串联重复和PAGE的柑橘黄龙病菌‘Candidatus Liberibacter asiaticus’种间遗传多样性分析(英文) 植物病理学报 2014,44 (6) 609-619
Position: Home > Articles > Intraspecific Genetic Diversity Analysis of ‘Candidatus Liberibacter asiaticus' by Short Tandem Repeats and PAGE Acta Phytopathologica Sinica 2014,44 (6) 609-619

基于短串联重复和PAGE的柑橘黄龙病菌‘Candidatus Liberibacter asiaticus’种间遗传多样性分析(英文)

作  者：
许美容;郑正;李昕昱;洪虹霞;邓晓玲
单  位：
广东省微生物信号与作物病害防控重点实验室;华南农业大学资源环境学院
关键词：
黄龙病;‘Candidatus Liberibacter asiaticus’;短串联重复;遗传多样性
摘  要：
韧皮部杆菌亚洲种(‘Candidatus Libriacter asiaticus’,‘Ca.L.asiaticus’)是目前流行范围最广、危害最严重的柑橘黄龙病致病菌。本研究利用‘Ca.L.asiaticus’基因组中全套串联重复基因(short tandem repeat,STR)开发了一套系统的方法用于‘Ca.L.asiaticus’遗传多样性和黄龙病分子流行学研究。PCR和PAGE分析筛选到的36个STR位点中,有33个获得了有效扩增。其中20对引物对不同来源的32个样品的扩增产物多态性较好,最多的可扩增出9种条带类型。采自我国6省的32个‘Ca.L.asiaticus’阳性样品之间的Shannon’s信息指数为0.14~1.90,平均为0.68;Nei’s基因多样性指数为0.06~0.81,平均为0.38,各省的菌株表现出较高的遗传多样性,特别是来自福建、广西和云南三省的。聚类分析发现可能为中国黄龙病发源地的广东省的黄龙病菌株具有一定的遗传特异性;其余邻省之间存在由木虱传播引起的菌株交流的可能性可以解释该病害在省际间传播。研究表明,开发的STR标记结合PAGE的方法可作为今后菌株遗传多样性和病害分子流行学分析的高效方法。
译  名：
Intraspecific Genetic Diversity Analysis of ‘Candidatus Liberibacter asiaticus' by Short Tandem Repeats and PAGE
作  者：
XU M ei-rong;ZHENG Zheng;LI Xin-yu;HONG Hong-xia;DENG Xiao-ling;Guangdong Key Laboratory of Microbial Signals and Disease Control,College of Resources and Environment,South China Agricultural University;
关键词：
Huanglongbing;;‘Candidatus Liberibacter asiaticus';;short tandem repeats;;genetic diversity
摘  要：
The most prevalent uncultured phloem-restricted bacterial species associated with citrus huanglongbing( HLB) disease in the world is ‘Candidatus Libriacter asiaticus'. To develop a set of short tandem repeat( STR) markers for‘Ca. L. asiaticus' and facilitate studies on its genetic diversity and epidemiology of HLB,Tandem Repeats Finder softw are was applied to identify and analyze the STR loci. STR primers w ere designed by Primer Premier 5. 0. Pop Gene and Pow er M ark w ere used to analyze polyacrylamide gel electrophoresis( PAGE) bands of isolates collected from 6 provinces in China. Thirty-three of the 36 STR loci could be effectively amplified. Tw enty polymorphic STR primers w ere selected and used to differentiate 32 Chinese ‘Ca. L.asiaticus' isolates. The Nei's measure of genetic diversity values for these 20 STRs ranged from 0. 06 to 0. 81,w ith a mean of 0. 38. The shannon's diversity indexes w ere betw een 0. 14 and 1. 90,w ith a mean of 0. 68. In conclusion,the 20 polymorphic STR loci w ere used for genetic diversity analysis of ‘Ca. L. asiaticus' isolates from different provinces in China. Diversity of ‘Ca. L. asiaticus' was related to geographic distribution. The isolates from Guangdong that w as considered to be HLB origin were specific. Isolates from Fujian,Guangxi and Yunnan were suggested to be higher in diversity. Psyllid transmission may play roles in the spread of‘Ca. L. asiaticus' between neighbor provinces.

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