ZHANG Gui-xue;LI Xiao-juan;YANG Ping;LIU Ying

rabbit spermatozoa;cryopreservation;extender;cryoprotectant

Glycerol, dimethyl suffixod (DMSO ) and acetamide were adopted to verify the cryoprotective ability of penetrating cryoprotectants with three kinds of extenders for rabbit sperm. Three extenders (A, B and C) and three level of the final concentrations of glycerol and DMSO (2%, 3% and 4%), and acetamide 2%, 4% and 5% were used with Extender A, 4% glycerol had better cryoprotective effect for rabbit sperm motility and 5% acetamide was better with Extender C. 4% acetamide was better in being combined with Extender A for sperm acrosome integrity ratio, 5% acetamide was better with Extender B and 2% acetamide was better with Extende C. With extender A, there was not difference among three penetrating cryoprotectants for plasma membrane integrity. With Extender B, 3% glycerol was better than 4% acetamide for plasma membrane integrity, and 3% glycerol with Extender C was better than 2% - 4% DMSO and 2% acetamide.