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当前位置: 首页 > 文章 > 甲酸盐和葡萄糖对两种土壤N_2O排放的刺激作用 农业环境科学学报 2019 (1) 235-242
Position: Home > Articles > Effect of formate and glucose organic carbon on N_2O emission from two soils Journal of Agro-Environment Science 2019 (1) 235-242

甲酸盐和葡萄糖对两种土壤N_2O排放的刺激作用

作  者：
马舒坦;颜晓元
单  位：
中国科学院大学;中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室
关键词：
根系分泌物;反硝化;真菌;细菌;N2O
摘  要：
为初步探究某些根系分泌物对土壤N_2O排放的影响,采用室内培养的方法,以甲酸盐和葡萄糖为外加碳源(0、0.5、1μmol C·g-1),测定了其对菜地和稻田土壤N_2O排放的影响。结果表明,无外加有机碳源情况下,菜地土壤的N_2O-N(2.65～2.69μg·kg-1)排放量显著小于稻田土壤(6.19～11.79μg·kg-1)(P<0.01);添加葡萄糖的情况下,菜地土壤的N_2O-N(2.47～3.44μg·kg-1)排放量也显著小于稻田土壤(9.55～13.34μg·kg-1)(P<0.01);但在甲酸盐(1μmol C·g-1)的刺激下菜地土壤N_2O-N排放量(54.86μg·kg-1)显著高于稻田土壤(42.40μg·kg-1)(P<0.01);增加施氮量并没有显著增加土壤中N_2O排放。荧光定量PCR结果表明,稻田土壤微生物拷贝数(包括真菌、细菌、反硝化细菌)是菜地的3～8倍。进一步通过高通量测序分析发现,反硝化真菌在菜地中的相对丰度(53.8%),远远高于其在稻田土壤中的丰度(6.6%)。有报道指出,反硝化真菌能够有效地利用甲酸盐产生N_2O,我们的研究也发现小分子有机物质的微量添加可以显著刺激土壤N_2O排放;微生物群落结构可以显著地影响土壤N_2O排放对不同有机碳源添加响应;甲酸盐添加下的菜地土壤中,大量反硝化真菌很可能是N_2O的主要贡献者。
译  名：
Effect of formate and glucose organic carbon on N_2O emission from two soils
作  者：
MA Shu-tan;YAN Xiao-yuan;State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences;University of Chinese Academy of Sciences;
单  位：
MA Shu-tan%YAN Xiao-yuan%State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences%University of Chinese Academy of Sciences
关键词：
root exudates;;denitrification;;fungi;;denitrifying fungi;;N2O
摘  要：
Incubation experiments were performed to preliminarily investigate the effects of some root exudates on soil N_2O emissions by adding different amounts of formate or glucose(0,0.5,1 μmol C·g-1)to vegetable field and paddy soils. In the absence of formate or glucose, the N_2O-N emissions(2.65～2.69 μg·kg-1)from the vegetable field soil were significantly less than those of the paddy soil(6.19～11.79μg·kg-1)(P<0.01). In the presence of glucose, N_2O-N emissions(2.47～3.44 μg·kg-1)from the vegetable field soils were also significantly less than those of the paddy soil(9.55～13.34 μg·kg-1)(P<0.01). Whereas, in the presence of 1 μmol C·g-1formate, the N_2O-N emissions(54.86 g·kg-1)from vegetable field soils were significantly larger than those of the paddy soil(42.40 g·kg-1)(P<0.01). The amount of NO-3addition didn′t significantly affect soil N_2O emissions. Quantitative PCR and high-throughput sequencing were used to explore the underlying microbial mechanism showing that microbial copy number of the paddy soil(including fungi, bacteria, denitrifying bacteria)was 3～8times higher than that of the vegetable field soil. According to the results of high-throughput sequencing analysis, the relative abundance of denitrifying fungi in vegetable field(53.8%)was much higher than that of the paddy soil(6.6%). It had been reported denitrifying fungi could utilize formate as electronic donor in the process of denitrification. Our results also indicated that low-molecular-weight organic carbon with trace addition significantly stimulated soil N_2O emission, and the effects depended on microbial community structure. Denitrifying fungi probably dominated N_2O emission when the vegetable field soil was amended with formate.

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