关键词：
竹加工剩余物;低聚木糖;乙醇;内切木聚糖酶;纤维素酶
摘 要：
以竹加工废弃物中纤维素、木聚糖组分高附加值利用为目的,采用内切木聚糖酶选择性降解竹屑木聚糖,制备高附加值产品低聚木糖,竹屑制备低聚木糖的废渣经糖化、发酵制备乙醇。采用7%的Na OH提取竹屑木聚糖1 h,木聚糖提取率为57.81%,竹屑木聚糖再在25 U/g内切木聚糖酶定向水解下,低聚木糖得率为39.49%,低聚木糖对青春双歧杆菌有良好的增殖作用。竹屑木聚糖抽提剩余物在25 U/g纤维素酶和4 U/gβ-葡萄糖苷酶条件下水解72 h,纤维素水解得率64.05%。121.20 g/L纤维素水解糖液经酿酒酵母NLH13发酵40 h,糖利用率和乙醇得率分别为98.80%和92.95%。研究结果可为竹加工废弃物的综合利用提供新的途径。
译 名：
Xylo-oligosaccharides and ethanol production from bamboo processing residues
作 者：
LI Han;LI Xin;XU Yong;CHEN Mu;YU Shiyuan;YONG Qiang;College of Chemical Engineering,Nanjing Forestry University;
关键词：
bamboo processing residues;;xylo-oligosaccharides;;ethanol;;endo xylanase;;cellulase
摘 要：
Xylo-oligosaccharides and ethanol preparation from bamboo processing residues were investigated. The bamboo xylan was selectively degraded by endo-xylanase to produce xylo-oligosaccharides. Xylan extraction residue was used for ethanol production through saccharification and fermentation processes. The xylan of bamboo processing residues was extracted with 7% Na OH solution for 1 h,the xylan extraction yield was 57. 81%. The xylan was hydrolyzed by endoxylanase with the dosage of 25 U / g xylan for 12 h,the xylo-oligosaccharides yield was 39. 49%,and the xylo-oligosaccharides have good effect on the proliferation of Bifidobacterium adolescentis. The xylan extraction residue was hydrolyzed by cellulase and β-glucosidase with the dosage of 25 U / g and 4 U / g for 72 h,64. 05% hydrolysis yield was obtained.The hydrolyzate containing 121. 20 g / L glucose was fermented by Saccharomyces cerevisiae NLH13 for 40 h,the glucose consumption and ethanol yield were 98. 80% and 92. 95%,respectively.
