摘 要：
利用含有1%盐酸的无水甲醇提取柿子果肉中的单宁,粗提液经大孔树脂AB-8初步纯化后,再经超滤膜法制得柿子单宁两级分:高分子量单宁(H igh M o lecu larW e igh t T ann in,HMW T)以及小分子量单宁(Sm a llM o lecu larW e igh t T ann in,SMW T)。HMW T具有良好的水溶性,凝胶渗透色谱(GPC)分析表明其分子量分布范围为1.16×104~1.54×104。抗氧化试验表明:HMW T对2-脱氧-D-核糖、甲基紫、水杨酸体系产生的羟基自由基均具有很强的清除能力,其最大清除率分别为90.47%、96.46%、87.77%,;HMW T对邻苯三酚自氧化和亚油酸脂质过氧化亦有较强的抑制作用,其最大抑制率分别为56.57%、69.63%。在所有体系中HMW T抗氧化能力呈明显的剂量效应关系,且效果均强于相同浓度的葡萄籽原花青素(OPC)及SMW T,同时表明HMW T是柿子单宁的主要抗氧化活性组分。
译 名：
Preparation and antioxidant activity of tannin from persimmon pulp
作 者：
Gu Haifeng1,Li Chunmei1,Xu Yujuan1,2,Zhang Jie1,Dou Hongliang1(1.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.Sericulture and Farm Produce Processing Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China)
关键词：
persimmon tannin;ultrafiltration fraction;antioxidant activity
摘 要：
Persimmon tannin was extracted from persimmon pulp with methanol acidified with 1% HCl,then AB-8 macroporous adhesive resin and polysulfone ultrafilter membrane with molecular weight cutoff of 10000 were employed to purify and fractionate the crude extract,and two fractions: Small Molecular Weight Tannin(SMWT) and High Molecular Weight Tannin(HMWT) were obtained.The molecular weight distribution of HMWT was determined to be in the range of 1.16×104 to 1.54×104.Antioxidant properties of HMWT were evaluated in 2-deoxy-D-Ribose system,methyl violet system,salicylic acid system,pyrogallol autoxidation system,and linoleic acid lipid peroxidation system.The maximum scavenging rate of HMWT on hydroxyl radical in 2-deoxy-D-Ribose,methyl violet and salicylic acid system were 90.47%,96.46% and 87.77%,respectively;the maximum inhibiting effect of HMWT on pyrogallol autoxidation and linoleic acid lipid preoxidation were 56.57% and 69.63%,respectively.HMWT exhibited excellent antioxidant activity in all tested sytems in a dose-dependent manner and the antioxidant activity of HMWT was significantly stronger than those of SMWT and grape seeds proanthocyanidins(OPC).This results suggest that HMWT is the main antioxidant in persimmon pulp.
