Construction and Detection of Streptomyces lydicus A01 Transformants with Elicitor Genes from Trichoderma

WU Qiong,CHEN Jie,LI Yingying,GAO Shigang,YU Chuanjin,LI Yaqian(Laboratory of Southen Urban Agriculture of Ministry of Agriculture/School of Agriculture & Biology,Shanghai Jiaotong University,Shanghai 200240,China)

Streptomyces lydicus A01;xyn2;sm1;conjugal-transformants

Trichoderma reesei QM 6a is characterized with a high level of XYN2 elicitor,which induces ethylene signaling pathway in plants,while sm1 elicitor produced by Trichoderma virens Gv29-8 is proved to induce jasmonic acid and ethylene signaling pathway.Both the elicitors could enhance the resistance of pathogens in plants.In this study,in order to improve the control effect of fungal pathogens by synergy of natamycin and elicitors,xyn2 and sm1 genes were synthetized according to the codon preference of Streptomyces coelicolor,linked into pIB139 expression vector and identified via enzyme digestion.The positive plasmid pIB139-xyn2(sm1) was transformed to ET12567(pUZ8002) intermediate strain and further into the genome of S.lydicus A01 by conjugal-transformed method, respectively.Then,for higher induce resistanc,S.lydicus A01 transformant with both xyn2 and sm1 was constructed. The results showed that: The three conjugal-transformants(A01-xyn2,A01-sm1,A01-xyn2+sm1) were constructed successfully,and they will have the synergistic effects of antagonism and induced resistance on plant disease.