Position: Home > Articles > AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR AVERMECTIN B_1 RESIDUE IN PEARS
Journal of Plant Protection
1996,23
(4)
333-337
梨中AVERMECTIN B_1残留的酶联免疫吸附测定
作 者：
李俊锁;钱传范
单 位：
中国农业大学应用化学系
关键词：
Avermectin B_1;梨;酶联免疫吸附测定(ELISA)
摘 要：
本文建立了检测梨中Avermectin B_1的间接竞争酶联免疫吸附测定法(ELISA)。以结合物4”—O—(单)琥珀酰Avermectin B_1—BSA免疫家兔,制备特异性针对Avermectin B_1的多克隆抗体。梨样本经甲醇提取后用ELISA检测。在6.0～60mg/kg添加浓度范围内,Avermectin B_1的回收率为86％～105％,变异系数(CV)为4％～8％。样本检测限为0.1mg/kg。本方法简便、快速、灵敏,适宜对大批量样本进行快速测定。
译 名：
AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR AVERMECTIN B_1 RESIDUE IN PEARS
作 者：
Li Junsuo Qian Chuanfan (Department of Applied Chemistry ,China Agricultural University,Beijing)
关键词：
Avermectin B1 , pears, ELISA
摘 要：
An indirect competitive enzyme-linked immunosorbent assay (ELISA) for determination of Avermectin B1 residue in pears is developed. The polyclonal anti-Aver-mectin B1 antibodies were obtained by immunizing rabbits with 4''-O-succinoylavermectin B1. The sample was extracted with methanol,diluted with PBST,and then monitored by ELISA. Recoveries of Avermectin B1 from pears fortified at 6. 0-60mg/kg levels were in a range of 86%-105% .with CV = 4%-8%. The limit of detection was 0. 1 mg/kg. This is a simple, sensitive and rapid method for monitoring Avermectin B1 residue in pears.
导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
