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当前位置: 首页 > 文章 > 凉山地区猪腹泻样本中PEDV、PDCoV和GARV感染检测 四川畜牧兽医 2017 (7) 18-20+22
Position: Home > Articles > Infection Dete Ction of PEDV,PDCoV and GARV in Pig Farms of Liangshan Prefecture Sichuan Animal & Veterinary Sciences 2017 (7) 18-20+22

凉山地区猪腹泻样本中PEDV、PDCoV和GARV感染检测

作  者：
彭艳伶;李昊;余琼;李建;张斌;沈思思;任玉鹏
单  位：
四川省西昌市农牧局;西昌学院动物科学院;西南民族大学生命科学与技术学院
关键词：
猪流行性腹泻病毒;猪Delta冠状病毒;猪A群轮状病毒;RT-PCR
摘  要：
为了解四川省凉山州部分猪场3种猪腹泻病毒的感染情况,本研究采用RT-PCR方法,分别对来自德昌、冕宁县和宁南县多个规模化猪场的60份猪腹泻样本进行病原检测,包括猪流行性腹泻病毒(PEDV)、猪Delta冠状病毒(PDCoV)和猪A群轮状病毒(GARV)。结果显示:3种病原的检出率分别为PEDV15%、PDCoV33.33%、GARV58.33%,其中冕宁县PDCoV检出率为65%,而GARV检出率更高达70%;3种病毒混合感染的情况普遍存在,总体混合感染率分别为:德昌15%(3/20),冕宁60%(12/20),宁南5%(1/20),混合感染类型中尤以PDCoV和GARV的混合感染率最高,提示PDCoV和GARV可能存在更高的协同致病性。
译  名：
Infection Dete Ction of PEDV,PDCoV and GARV in Pig Farms of Liangshan Prefecture
作  者：
Peng Yanlin;Li Hao;Zhang Bin;Agriculture and Animal Husbandry Bureau of Xichang City;Animal Science College of Xichang University;College of Life Science and Technology,Southwest University for Nationalities;
关键词：
PEDV;;PDCoV;;GARV;;RT-PCR
摘  要：
To investigate the infection of PEDV, PDCoV and GARV from different pig farms in 3counties of Liangshan prefecture,this study detected three kinds of pathogens from a total of 60 feces samples by RT-PCR method. The results showed that the detection rates of these three kinds of pathogens were PEDV 15%, PDCoV 33.33%, GARV 58.33%, respectively. It indicated that PEDV, PDCo V and GARV were widespread in pig farms of Liangshan prefecture. Besides, the positive rate of PDCo V in Mianning county was 65%,which was significantly higher(P<0.01) than that of the others,and the detection rate of GARV in this area was 70%. Moreover, These three pathogens often presented mixed infection, and the mixed infection rates in different areas were Dechang 15%(3/20), Mianning 60%(12/20), Ningnan 5%(1/20),respectively. Within these mixed infection types,the mixed infection rate of PDCoV and GARVwas highest,it could be inferred that GARV and PDCoV might have synergistic pathogenicity.

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