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当前位置: 首页 > 文章 > 不同性腺激素水平对子宫内膜细胞体外增殖的影响 畜牧兽医学报 2006,37 (10) 987-991
Position: Home > Articles > Effect of Different Level of Sex Steroids on the Proliferation of Endometrial Cells in vitro Chinese Journal of Animal and Veterinary Sciences 2006,37 (10) 987-991

不同性腺激素水平对子宫内膜细胞体外增殖的影响

作  者：
宋宇轩;曹斌云;王建刚;李胜;程雪妮;朱广琴
单  位：
陕西省畜牧兽医总站;西北农林科技大学动物科技学院
关键词：
子宫内膜;上皮细胞;基质细胞;性腺激素
摘  要：
为探讨不同雌激素(E2)和孕激素(P4)水平对家兔子宫内膜细胞体外增殖和分化的影响。采用离心法获取家兔子宫内膜上皮细胞及基质细胞,设不同雌激素和孕激素水平组合添加于培养液,利用MTT法间接测定不同性腺激素水平对子宫上皮和基质细胞的体外增殖的影响;利用免疫组织化学法检测传代细胞是否分化为其他类型细胞。结果表明利用离心法可获得纯度较高的子宫内膜上皮和基质细胞;孕激素(100 nmol/L)对基质细胞的增殖有促进作用(P<0.05),雌激素(100 nmol/L)和少量孕激素(10 nmol/L)对上皮细胞增殖有促进作用(P<0.05);免疫组化结果表明,经性腺激素刺激的子宫内膜上皮和基质细胞分别对上皮角蛋白抗体和波形蛋白抗体呈阳性。研究证实适宜的性腺激素水平对体外培养的子宫内膜细胞有促进增殖的作用,而且不会促使其分化为其他类型细胞。
译  名：
Effect of Different Level of Sex Steroids on the Proliferation of Endometrial Cells in vitro
作  者：
SONG Yu-xuan~(1),CAO Bin-yun~(*1),WANG Jian-gang~1,LI Sheng~2,CHENG Xue-ni~1,ZHU Guang-qin~1 (1.College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;2.The General Station of Animal Husbandry and Vetrinary of Shanxi Province,Xian 710016,China)
关键词：
endometrium;epithelial cell;stromal cell;sex steriods
摘  要：
To study the effect of different level of sex steroids on the proliferation and differentiation of endometrial cells,rabbit endometrial epithelial cells and stromal cells were isolated using centrifugation method.The different level and combination of progesterone(P_4) and β-estradiol(E_2) were added in media,the proliferative capability of the cells in vitro was determined indirectly by MTT method.The specificity and homogeneity of the epithelial cells and stromal cell cultured in media containing sex steroids were determined by immunocytochemistry employing antibodies against cytokeratin and vimentin respectively.The results stated that high purity epithelial cells and stromal cells were isolated using centrifugation method.100 nmol/L P_4 in media can stimulate the proliferation of stroma cell,thus a combination of 100 nmol/L E_2 and 10 nmol/L P_4 in media can stimulate the proliferation of epithelial cell(P<0.05).Immunocytochemistry staining demonstrated that cytokeratin was positive in epithelial cells and vimentin was detectable in stromal cells.This study demonstrated that sex steroids can promote proliferation of endometrial cells in vitro and maintain its specificity and homogeneity.

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