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当前位置: 首页 > 文章 > 中国荷斯坦牛LYZ基因多态性及其与乳房炎的关联分析 中国农业科学 2010,43 (23) 4936-4941
Position: Home > Articles > Polymorphism of LYZ Gene and Its Association with Mastitis Trait in Chinese Holstein Scientia Agricultura Sinica 2010,43 (23) 4936-4941

中国荷斯坦牛LYZ基因多态性及其与乳房炎的关联分析

作  者：
陈仁金;杨章平;毛永江;陈莹;常玲玲;吴海涛;冀德君;李云龙;张亚琴
单  位：
扬州大学动物科学与技术学院
关键词：
中国荷斯坦牛;LYZ基因;PCR-SSCP;多态性;体细胞评分;305d产奶量;乳房炎抗性
摘  要：
【目的】通过研究中国荷斯坦牛溶菌酶基因(lysozyme,LYZ)编码区多态性与乳房炎的关联性,寻找与乳房炎相关的分子标记,加快中国荷斯坦牛的抗病育种进程。【方法】利用PCR-SSCP技术对30个公牛家系的610头中国荷斯坦牛LYZ基因进行多态性检测,利用最小二乘均数法对LYZ基因的多态位点与SCS和305d产奶量进行相关分析【结果】LYZ基因的外显子1上存在115(T>G)突变位点,突变使精氨酸变为亮氨酸;突变位点经PCR-SSCP法检测发现了3种基因型TT、TG和GG,频率分别为0.270、0.508和0.222,χ2检验表明中国荷斯坦牛在该位点偏离Hardy-Weinberg平衡;GG基因型个体的SCS最小二乘均值极显著低于TT基因型个体(P<0.01),显著低于TG基因型个体(P<0.05);GG基因型个体的305d产奶量最小二乘均值极显著高于TT基因个体(P<0.01),显著高于TG基因型个体(P<0.05)。【结论】115(T>G)位点GG基因型,对中国荷斯坦牛的SCS和305d产奶量有较大的遗传效应,可作为分子标记应用于奶牛乳房炎抗性的筛选。
译  名：
Polymorphism of LYZ Gene and Its Association with Mastitis Trait in Chinese Holstein
作  者：
CHEN Ren-jin,YANG Zhang-ping,MAO Yong-jiang,CHEN Ying,CHANG Ling-ling,WU Hai-tao,JI De-jun,LI Yun-long,ZHANG Ya-qin(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,Jiangsu)
关键词：
Chinese Holstein;LYZ gene;PCR-SSCP;polymorphism;SCS;305 d milk yield;mastitis resistance
摘  要：
【Objective】 The polymorphism of coding region of LYZ gene and its correlation with somatic cell score(SCS) and 305 d milk yield in Chinese Holstein were analyzed for the purpose of providing molecular markers in the marker-assisted selections(MAS) to accelerate the disease-resistant breeding progress in Chinese Holstein.【Method】 The polymorphism of LYZ gene was detected by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) technique in 610 Chinese Holstein cattle from 30 bull families.The associations between polymorphic sites of LYZ gene and SCS and 305 d milk yield were analyzed by the least square mean analysis.【Result】 The 115(T>G)mutation site was found in the exon1,and the mutation resulted in an amino acid change of Arg→Leu,for the polymorphic site,three genotypes(TT,TG and GG) were found in 610 Chinese Holstein by PCR-SSCP,and the frequency of TT,TG and GG genotypes was 0.270,0.508 and 0.222,respectively.Chi-square tested showed Chinese Holstein was not in Hardy-Weinberg equilibrium at the site.Least square mean of SCS for GG genotype was highly and significantly lower than that for TT(P<0.01),and significantly lower than TG genotype(P<0.05).Least square mean of 305 d milk yield for GG genotype was greatly and significantly higher than that for TT(P<0.01),and significantly higher than TG genotype(P <0.05)【.Conclusion】 GG genotype at 115(T>G)site had a great genetic effect on SCS and 305 d milk yield in Chinese Holstein,which can be used as a candidate marker for mastitis resistance selection in dairy cattle.

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