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Position: Home > Articles > Cloning and Functional Analysis of a IpLEA(Late embryogenesis abundant)Gene from Ipomoea pes-caprae Molecular Plant Breeding 2018 (13) 4235-4244

厚藤胚胎发生晚期丰富蛋白基因IpLEA的克隆与功能分析

作者：

郑洁旋;张会;简曙光;夏快飞;张美

关键词：

厚藤;LEA基因;逆境胁迫

摘要：

LEA(Late embryogenesis abundant)基因是生物体中广泛存在的一类广泛响应逆境胁迫的功能基因,主要参与调控植物对缺水等逆境胁迫的耐受。为了探索厚藤LEA基因的抗逆分子机制,本研究通过前期对厚藤(Ipomoea pes-caprae L.)c DNA文库的筛选,获得了一个编码厚藤LEA基因的全长c DNA,命名为IpLEA(Gen Bank登录号:MF680612)。通过在酵母中表达,表明IpLEA能够提高转基因酵母的耐盐性及抗氧化能力。以厚藤成年植株及幼苗为材料进行实时荧光定量RT-PCR分析的结果表明:IpLEA基因在厚藤各组织中广泛表达;在厚藤幼苗中的表达受到高盐、甘露醇胁迫和ABA处理的诱导。推测IpLEA基因参与厚藤应答高盐、干旱等逆境胁迫,并响应ABA信号的传递。本研究为今后厚藤IpLEA基因的进一步功能发掘和厚藤响应极端逆境的机理研究提供了理论依据。

译名：

Cloning and Functional Analysis of a IpLEA(Late embryogenesis abundant)Gene from Ipomoea pes-caprae

作者：

Zheng Jiexuan;Zhang Hui;Jian Shuguang;Xia Kuaifei;Zhang Mei;Provincial Key Laboratory of Applied Botany,South China Botanical Garden;University of Chinese Academy of Sciences;

单位：

Zheng Jiexuan%Zhang Hui%Jian Shuguang%Xia Kuaifei%Zhang Mei%Provincial Key Laboratory of Applied Botany,South China Botanical Garden%University of Chinese Academy of Sciences

关键词：

I.pes-caprae;;LEA gene;;Adversity stress

摘要：

LEA(Late embryogenesis abundant) gene is a functional gene widely responding to adversity stress which generally exists in living organisms, and is mainly involved in regulating the tolerance of plants to stresses such as water shortage. Through the screening of cDNA library of Ipomoea pes-caprae in early stage, the study obtained a full length c DNA encoding LEA protein of I. pes-caprae, and named as IpLEA(Gen Bank accession number: MF680612) with the purpose to reveal the stress resistance mechanism of LEA gene in I. pes-caprae. The expression of IpLEA in yeast indicated that IpLEA could improve the salt tolerance and antioxidant ability of transgenic yeast. Using adult plants and seedlings of I. pes-caprae as materials to carry out real-time fluorescence quantitative RT-PCR analysis, the result showed that IpLEA gene was widely expressed in various tissues in I.pes-caprae, and the expression in seedling was induced under high salt, mannitol stress, and ABA treatment. It was speculated that IpLEA gene might play important roles in responding to adversity stresses such as high salt and drought, and ABA signal transmission. This study might provide a theoretical basis for further functional excavation of IpLEA genes and the mechanisms of responding to extreme adversity in I. pes-caprae.

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厚藤胚胎发生晚期丰富蛋白基因IpLEA的克隆与功能分析

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