当前位置: 首页 > 文章 > 布尔山羊和布奶杂一代山羊超数排卵试验 动物医学进展 1999 (2)
Position: Home > Articles > Superovulation of Boer goat and F1 hybrids of Boer and Guanzhong milk goat Progress in Veterinary Medicine 1999 (2)

布尔山羊和布奶杂一代山羊超数排卵试验

作  者:
王希朝;王光亚;赵晓娥
单  位:
西北农业大学动物科学与动物医学学院
关键词:
山羊,超数排卵,胚胎移植
摘  要:
7只布奶杂一代山羊和1只布尔山羊,阴道放置海绵栓同时注射雌二醇2mg预处理7d,随机分为2组,开始超排处理。第1组每日上午肌注用生理盐水溶解的FSH,连续4d,剂量分别为150,100,50,50IU。第2组一次肌注用300g/LPVP溶解的FSH250~300IU。两组均于处理后的第3天下午撤除阴道海绵栓,同时皮下注射15-甲基PGF2α0.6mg,第1组于第4天上午再次注射15-甲基PGF2α0.6mg。发情后人工授精2~3次,每次3粒冷冻精液,间隔8h。发情配种后的第5天进行腹腔镜检查,第6天手术回收胚胎。第1组山羊平均回收可用胚9.5枚,第2组为10.3枚,两组回收的可用胚差异不显著。说明在放置海绵栓时配合应用雌二醇,并缩短放置时间有改善超排效果的作用。用PVP溶液溶解FSH一次注射与常用的多次注射的超排效果没有差异,简化了超排程序,使山羊的胚胎移植更便于在生产中应用。
译  名:
Superovulation of Boer goat and F1 hybrids of Boer and Guanzhong milk goat
作  者:
Wang Xichao Wang Guangya Zhao Xiaoe (College of Animal Science and Veterinary Medicine Northwestern Agricultural University ,Yangling, Shaanxi 712100)
关键词:
goat,superovulation,embryo transfer
摘  要:
Eight goats including seven F1 hybrids of Boer goat and Guanzhong milk goat and one Bore goat were inserted vaginal sponges and injected with estradiol benzoate 2 mg.After 7 days,these goats were divided into two groups at random and began to superovulation.The first group was muscle injected with FSH dissolved in physiological saline solution in every morning for 4 days.The doses were 150,100,50, 50 IU respectively .The second group was single muscle injected with FSH 250 ̄300 IU dissolved in 300 g/L polyvinylpyrrolidone (PVP).Two groups were removed vaginal sponges and injected with 15methylprosta glandin F2(PGF2)0.6 mg under skin 3 days after treatment. The first group was injected again in fourth morning .Goats were fertilized through artificial insemination after estrus.Embryo recovered by surgery at sixth day.The average number of useful embryos is 9.5 from the former group while 10.3 from the latter .The difference between the numbers is not significant(P>0.05).This showed that it can improve superovulation result if goat is inserted with vaginal sponges combined with using estradiol,applying time of vaginal sponges is shortened as well.The single injection with FSH dissolved in PVP makes superovulation program simply and get embryos transfer of goats more feasible in production.
计量
文章访问数: 1
HTML全文浏览量: 0
PDF下载量: 0

所属期刊