当前位置: 首页 > 文章 > 双水相法提取木聚糖酶的初步研究 食品科学 2009,30 (5) 214-216
Position: Home > Articles > Study on Aqueous Two-phase Extraction of Xylanase FOOD SCIENCE 2009,30 (5) 214-216

双水相法提取木聚糖酶的初步研究

作  者:
苏玉春;陈光;白晶;韩微微;王中兴
单  位:
吉林农业大学生命科学学院
关键词:
双水相法;木聚糖酶;纯化
摘  要:
采用双水相法从黑曲霉AS3.4309的发酵液中提取木聚糖酶,通过正交试验对双水相的组成进行优化。结果表明,双水箱体系最佳组成为:选用PEG4000,其浓度为19%(W/W),磷酸氢二钾浓度为10%(W/W),氯化钠浓度为0(W/W)。此条件下,木聚糖酶的提取效果较好,分离系数(K)和上相产率(Yt)分别为6.23和88.67%。
译  名:
Study on Aqueous Two-phase Extraction of Xylanase
作  者:
SU Yu-chun,CHEN Guang,Bai Jing,HAN Wei-wei,WANG Zhong-xing (College of Life Science, Jilin Agricultural University, Changchun 130118, China)
关键词:
aqueous two-phase extraction;xylanase;purification
摘  要:
Orthogonal tests were designed to optimize the aqueous two-phase extraction process of xylanase from fermentation broth of Aspergillus niger AS3.4039 in this study. The results indicated that the optimum concentrations of PEG 4000, K2HPO4 and NaCl are 19%, 10% and 0 (W/W), respectively. Under these conditions, the separation factor (K) and xylanase yield in top phase (Yt) are 6.23 and 88.67% respectively, and the purification effect is satisfying.

相似文章

计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 2

所属期刊

推荐期刊