An electrochemical immunosensor for ultrasensitive detecton of alpha-fetoprotein based on nitrogen-doped porous carbon and PoPD

Gao Ke;Sun Zhao;Cao Kaihang;Yang Yuxiao;Qiao Xiuwen;Li Hongling;Qi Yu;Ye Bangce;College of Chemistry and Chemical Engineering,Shihezi University/Key Laboratory for Chemical Material of Xinjiang Uygur Autonomous Region/Engineering Center for Chemical Materials of Xinjiang Bingtuan;

POPD;;prous carbon;;N-doped;;alpha-fetoprotein;;immunosensor

In this study, we constructed a label-free electrochemical immunosensor for highly selective detection, stability and sensitive detection of alpha-fetoprotein(AFP) based on PoPD and N-doped porous carbon. The electrochemical characteristics with the modified electrode surface were studied by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS)measurements. The using of N-doped porous carbon greatly enhanced the biology catalytic activity due to its large specific surface and excellent biocompatibility. PoPD has large amino which can improved its ability to fixed anti-AFP. Under the optimized conditions, the results show that the immunosensor have excellent electrochemical properties with a wide concentration range from 0.005 to 100 ng/mL and a detection limit of 0.0006 ng/mL. The new type of electrochemical immunosensor may provide potential applications for the clinic application.