当前位置: 首页 > 文章 > 悬浮培养红豆杉细胞分裂同步化诱导 湖北农学院学报 2000,20 (2) 161-163
Position: Home > Articles > Induction of Mitotic Synchronization in Suspension Culture of Taxus chinensis Cells Journal of Hubei Agricultural College 2000,20 (2) 161-163

悬浮培养红豆杉细胞分裂同步化诱导

作  者:
刘华;钟阳
单  位:
华中师范大学生命科学院;湖北省卫生职工医学院
关键词:
红豆杉细胞;细胞培养;低温;咖啡因;有丝分裂指数;同步化
摘  要:
分别采用低温和咖啡因对红豆杉 (Taxuschinensis)细胞进行处理 ,诱导细胞分裂同步化。结果显示 ,4℃处理 2 4h效果最好 ,分裂指数提高 1 1 5.7%,χ2 =1 6.82 0 ,显著性极高。 8℃处理一定时间分裂指数也可显著提高。而 1 5℃处理效果最差。用高浓度的咖啡因处理较短时间也可有效诱导同步化
译  名:
Induction of Mitotic Synchronization in Suspension Culture of Taxus chinensis Cells
作  者:
LIU Hua (Hubei Medical Staff College,Jingzhou ,Hubei 434000,China) ZHONG Yang (College of Biological Science,Central China Normal University,Wuhan,Hubei 430079,China)
关键词:
cells ofTaxus chinensis;cell culture ;low temperature; caffeine; mitotic index; synchronization
摘  要:
Taxus chinensis cells were treated with low temperature and caffeine in order to induce their mitotic synchronization.The results indicated:when the temperature was set at 4 ℃ for 24 hours,the effect was the best,the mitotic index was extremely significantly increased to 115.7%(χ 2=16.820);when the temperature was set at 8 ℃ for a certain period of time,the mitotic index was increased significantly;while the temperature was set at 15 ℃,the effect was the worst.If these cells were treated with caffeine of high concentration for a short period of time,their mitosis could also be synchronized.

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