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Position: Home > Articles > Establishment and application of real-time PCR method for detection of rabbit haemorrhagic disease virus Heilongjiang Animal Science and Veterinary Medicine 2013 (3) 95-97

检测兔出血症病毒荧光定量方法的建立与应用

作  者:
刘行波;原东伟;刘家森;郭冬春;姜骞
单  位:
黑龙江八一农垦大学动物科技学院中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/自然疫源性人兽共患病研究团队;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;黑龙江八一农垦大学动物科技学院中国农业科学院;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室实验动物中心
关键词:
兔出血症病毒(RHDV);vp60;real-time PCR;隐性感染
摘  要:
为了研究兔出血症病毒(Rabbit hemorrhagic disease virus,RHDV)在实验动物兔和野兔中的发生情况并能更精确检测该病毒,试验采用建立的SYBR Green荧光定量方法对10只黑龙江省各单位送检的实验动物兔和5只哈尔滨郊县疑似病例的脏器样品进行了检测。结果表明:该方法有较好的灵敏度和特异性,能够用于兔出血症病毒的检测。
译  名:
Establishment and application of real-time PCR method for detection of rabbit haemorrhagic disease virus
作  者:
LIU Xing-bo1,2,YUAN Dong-wei2,LIU Jia-sen2,GUO Dong-chun2,JIANG Qian2, LIN Huan2,SI Chang-de2,CUI Yu-dong1,QU Lian-dong2(1.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319, China;2.Division of Experimental Animal Research,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academiy of Agriculture Science,Harbin 150001,China)
关键词:
Rabbit hemorrhagic disease virus(RHDV);vp60;real-time PCR;inapparent infection
摘  要:
To detect Rabbit hemorrhagic disease virus(RHDV)more precisely and learn the occurrences of this virus between experimental rabbits and hare.A real-time PCR method with SYBR Green was established for the detection of ten experimental rabbits from the various units in Heilongjiang Province for inspection and 5 samples of suspected cases in hares.The results indicated that the method had good sensitivity and specificity,and could be used to detect RHDV.

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