中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 拟南芥CBL3与CIPK9在花粉管中的相互作用 分子植物育种 2019 (16) 5320-5325
Position: Home > Articles > Interaction between Arabidopsis CBL3 and CIPK9 in Pollen Tube Molecular Plant Breeding 2019 (16) 5320-5325

拟南芥CBL3与CIPK9在花粉管中的相互作用

作  者：
周利明;房玮
单  位：
华北理工大学生命科学学院
关键词：
花粉管;顶端生长;钙传感器;信号通路
摘  要：
Ca2+是调控花粉萌发和花粉管生长的重要第二信使,然而目前只有少数信号组分被证实参与花粉管生长中的Ca2+信号途径。本研究通过酵母双杂交及荧光共振能量转移技术验证了Ca2+传感器CBL3与其下游效应子CIPK9之间存在着相互作用,而缺少C末端的CIPK9ΔC无法与CBL3发生互作。CIPK9-GFP定位于花粉管中的颗粒状细胞器,CBL3的加入可以将其改变为质膜及内膜系统定位,这一亚细胞定位与CBL3-GFP的定位相似。另外缺少C末端的CIPK9ΔC在与CBL3共同表达的情况下表现出胞内的弥散定位,说明CIPK9的C末端是CBL3与CIPK9相互作用的关键区域,CBL3与CIPK9的互作有助于CIPK9定位于花粉管质膜与内膜系统。
译  名：
Interaction between Arabidopsis CBL3 and CIPK9 in Pollen Tube
作  者：
Zhou Liming;Fang Wei;College of Life Sciences, North China University of Science and Technology;
关键词：
Pollen tube;;Tip growth;;Calcium sensor;;Signaling pathway
摘  要：
Calcium acts as an important second messenger that modulates pollen germination and pollen tube tip growth. However, up to date, only a few signaling components have been identified to recognize and transmit Ca2+signaling in pollen tube tip growth. The interaction between Ca2+sensor CBL3 and its downstream effector CIPK9 was verified by yeast two-hybrid and fluorescence resonance energy transfer(FRET). CIPK9ΔC without C-terminal could not interact with CBL3. CIPK9-GFP was localized to granular organelles in pollen tube. The addition of CBL3 changed CIPK9-GFP localization pattern into plasma membrane(PM) and endomembrane system. This localization pattern was similar to that of CBL3-GFP in pollen tube. In addition, CIPK9ΔC without C-terminal exhibits intracellular diffusion localization in pollen tube when co-expressed with CBL3. These results suggest that the C-terminal of CIPK9 is the key region for the interaction between CBL3 and CIPK9, which contributes to PM and endomembrane localization of CIPK9 in pollen tubes.

相似文章

导出引用
计量
文章访问数: 67
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46