中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 高致病性猪蓝耳病自然病例血清致炎因子IL-1、IL-6和TNF-α含量的测定与分析 中国动物检疫 2016 (1) 67-70
Position: Home > Articles > Detection and Analysis of IL-1、IL-6 and TNF-α in Sera of Natural HP-PRRS Infected Pigs China Animal Health Inspection 2016 (1) 67-70

高致病性猪蓝耳病自然病例血清致炎因子IL-1、IL-6和TNF-α含量的测定与分析

作  者：
李建臻;杨苗;徐刚
单  位：
成都农业科技职业学院;四川出入境检验检疫局
关键词：
高致病性蓝耳病;自然病例;IL-1;IL-6;TNF-α
摘  要：
本试验旨在探究高致病性蓝耳病(HP-PRRS)自然病例中致炎因子IL-1、IL-6和TNF-α含量,进而阐述HP-PRRS的致病机理,为养殖生产中的HP-PRRS防控提供理论基础。在成都地区4个发病猪场采集具有临床症状的猪血清(含5头濒死猪)35份与无临床症状猪血清23份,利用RT-PCR分别检测出HP-PRRSV阳性血清25份和11份。将检测出阳性表现症状的25份血清、检测阳性无临床症状的11份血清及检测阴性的12份血清分成3组,分别标注为病猪组、隐性感染组和健康对照组,运用ELISA方法进行IL-1、IL-6和TNF-α含量测定。结果显示,IL-1含量在三组中差异极显著(P<0.01),病猪组极显著高于隐性感染组与健康组(P<0.01),隐性感染组与健康对照组差异不显著(P>0.05);IL-6含量在三组中差异显著(P<0.05),病猪组显著高于隐性感染组与健康对照组(P<0.05),隐性感染组与健康对照组差异不显著(P>0.05);TNF-α含量在三组之间无显著差异(P>0.05)。该结果表明IL-1与IL-6在HP-PRRS感染致病中具有重要作用。
译  名：
Detection and Analysis of IL-1、IL-6 and TNF-α in Sera of Natural HP-PRRS Infected Pigs
作  者：
Li Jianzhen;Yang Miao;Xu Gang;Chengdu Agricultural Science and Technology Vocational College;Sichuan Entry Exit Inspection and Quarantine Bureau;
关键词：
HP-PRRS;;natural infection;;IL-1;;IL-6;;TNF-α
摘  要：
To find the relationship among IL-1、IL-6、TNF-α content in blood sera of HP-PRRSV infected pigs,so to explain the pathogenesis of HP-PRRS,and to provide a theoretical basis for the prevention and control of HP-PRRS,48 blood sera samples from 4 farms in Chengdu were sampled and tested by ELISA,including 25 RT-PCR positive samples with clinical symptoms and 11 positive samples without clinical symptoms and 12 negative samples.The results showed that the difference of IL-1 in positive pigs was significant(P<0.01)compared with the other two groups,and the differences of IL-1 between the other two groups were not significant(P>0.01).The differences of IL-6 levels among the three groups were significant(P<0.05),and IL-6 in positive samples with clinical symptoms were higher(P<0.05)than the other two groups.The differences of of IL-6 in the other two groups with negative results were not significant(P>0.05).The differences of TNF-α among the three groups were not significant(P>0.05).These results showed that IL-1and IL-6 increased in blood serum when pigs were infected by HP-PRRSV and played important role in infection,while TNF-α has little change.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46