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Position: Home > Articles > The Effect of β-Glucanase in Degradation Pachymaran Northern Horticulture 2011 (24) 18-22

β-葡聚糖酶降解茯苓多糖的效应

作  者:
郭雨桐;肖文军
单  位:
湖南农业大学园艺园林学院;国家中医药管理局亚健康干预技术实验室湖南农业大学
关键词:
茯苓;茯苓多糖;β-葡聚糖酶;酶促降解;提取
摘  要:
以资源丰富且多糖含量高的茯苓为原料,以茯苓多糖的提取得率为指标,通过酶促降解的单因子试验、正交实验及验证试验,研究了β-葡聚糖酶对茯苓多糖的降解效应,优化筛选了β-葡聚糖酶降解茯苓多糖的技术参数。结果表明:反应温度55℃、时间90min、pH 5.5、酶浓度6.5%是β-葡聚糖酶降解茯苓多糖的最优技术参数,在该参数条件下,应用β-葡聚糖酶提取茯苓多糖的得率为13.31%,对茯苓多糖的高值化利用具有较好的参考指导价值。
译  名:
The Effect of β-Glucanase in Degradation Pachymaran
作  者:
GUO Yu-tong1,2,XIAO Wen-jun1(1.College of Horticulture and Landscape,Hunan Agricultural University,Changsha,Hunan 410128;2.State Administration of Traditional Chinese Medicine Sub-health Intervention Technology Laboratory,Hunan Agricultural University,Changsha,Hunan 410128)
关键词:
Poria cocos;Poria cocos polysaneharide;β-glucanase;enzymatic degradation;extract
摘  要:
With the single-factor test,orthogonal experiment and verification test on enzyme dosage,enzymolysis time,enzymolysis temperature and pH value,using resource-rich,high-polysaccharide Poria cocos as the raw material and extraction rate of pachymaran as the comparative standard,the β-glucanase effective on the degradation of pachymaran systematically and optimized the selection of the β-glucanase's technical parameters on the degradation of pachymaran were studied.The results showed that when the pH was 5.0,the enzyme dosage was 0.13 g(6.5%),the enzymolysis temperature was 55℃,and the enzymolysis time was 90 min,the extraction rate of pachymaran,using the β-glucanase was 13.31%.It was significantly superior to the traditional yield of pachymaran.

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