单 位：
延边州农委;延吉市农业局;延边大学农学院
关键词：
等位基因特异性扩增;雨久花;乙酰乳酸合成酶基因;抗药性;检测
摘 要：
等位基因特异性扩增(Allele-specific PCR简称:ASPCR)技术是一项快速、简便、低成本的检测等位基因单核苷酸多态性的新方法。作者针对雨久花感抗磺酰脲类生态型ALS基因编码区第592位碱基密码(ALS氨基酸第197位)特征,采用在引物3′末端设有胸腺嘧啶核苷酸(感性)或鸟嘌呤(抗性)核苷酸的2种引物,对延边稻田发生的雨久花感抗磺酰脲类生态型ALS基因编码区第592位进行了ASPCR。检测结果显示,当以引物3′末端设有胸腺嘧啶引物时,感性类型出现ASPCR扩增带,而抗性类型无带出现,反之抗性类型有带而感性类型无带,检测到延边稻田发生的抗磺酰脲类雨久花生态型的ALS 197位点的脯氨酸被组氨酸替代,证明了该方法的有效性和可靠性。
译 名：
Development of allele-specific PCR for detection of herbicide-resistance in Monochoria korsakowii Regel et Maack
作 者：
WU Ming-gen1,JIN Wan-he2,XU Yong-nan3 (1.Agricultural College,Yanbian University,Yanji 133000;2.Agricultural Office of Yanbian,Yanji 133000; 3.Agricultural Bureau of Yanji,Yanji 133000)
关键词：
Allele-specific PCR;Monochoria korsakowii Regel et Maack;ALS gene;herbicide-resistance;detection
摘 要：
Allele-specific PCR is a rapid,simple,and low-cost method for single-nucleotide polymorphism identification.Using this technique,a single-nucleotide polymorphism primer marker of thymine(susceptible) or guanine(resistant) at the 3′end on 592 point of ALS(acetolactate synthetase) gene encoding site(197 point of ALS amino acid) was designed to detect the herbicide-resistant type of Monochoria korsakowii Regel et Maack.in Yan-bian region.The result shows that susceptibility primer marker can be used to detect the susceptibility biotype;otherwise can be detected resistant biotype;the cause of resistance to sulfonylurea herbicide type can be induced by substitution of Pro197 to histidine.It proved that the technique was efficient and feasible to detect ALS gene of herbicide-resistant type.
