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三唑类农药及其金属配合物对病原真菌抑制效果研究

作  者:
方利敏;汪流伟;熊丹;聂旭亮;戴金岳;姚明印;黄林
单  位:
江西农业大学工学院;江西农业大学生物科学与工程学院/江西省农业微生物资源开发与利用工程实验室/应用微生物研究所;江西农业大学理学院
关键词:
三唑类农药;金属配合物;广谱性;抑菌活性
摘  要:
为评价三唑类农药及其金属配合物对病原菌的抑制效果。试验采用滤纸片法测定3种三唑类农药及其金属配合物对6种病原真菌、2种细菌以及2种酵母菌抑制作用,选取抑菌谱较广且效果较好的药剂,经菌丝生长速率法和牛津杯法进一步测定其对松球壳孢菌、西瓜枯萎病菌、黑曲霉、桔青霉和意大利青霉等病原真菌的抑菌效果。结果表明,烯唑醇Co(Ⅱ)配合物抑菌广谱性更强,其对上述真菌、细菌及酵母菌均具有抑制作用,尤其对病原真菌的抑制效果最好,当浓度为2.50 mg/mL时,对松球壳孢菌的抑制率达到95.81%,此时意大利青霉的抑菌圈直径达到了31.77 mm。而烯唑醇Ag配合物的针对性更强,其对松球壳孢菌和意大利青霉菌的抑制作用最强,当浓度为0.50 mg/mL时,能完全抑制松球壳孢菌菌丝的生长,此时意大利青霉的抑菌圈直径为25.07 mm,抑制效果明显强于黑曲霉和桔青霉。可见烯唑醇Co(Ⅱ)和烯唑醇Ag配合物对病原真菌均有较好的抑菌效果,具有较高的应用潜力。
译  名:
Study on Antibacterial Effect of Triazole Fungicides and Their Metal Complexes on Pathogenic Fungi
作  者:
FANG Li-min;WANG Liu-wei;XIONG Dan;NIE Xu-liang;DAI Jin-yue;YAO Ming-yin;HUANG Lin;Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources/Institute of Applied Microbiology/College of Bioscience and Bioengineering, Jiangxi Agricultural University;College of Science, Jiangxi Agricultural University;College of Engineering, Jiangxi Agricultural University;
关键词:
triazole fungicides;;metal complexes;;broad spectrum;;anti-bacterial activity
摘  要:
To evaluate the inhibitory effect of triazole fungicides and their metal complexes on micro-organisms, experiments with the filter paper method were carried out to determine the inhibitory effects of 3 triazole fungicides and their metal complexes on six pathogenic fungi, two kinds of bacteria and two kinds of yeasts. The fungicides with wide antimicrobial spectrum and good inhibitory effects were selected to determine the inhibiting effects on pathogenic fungi such as Sphaeropsis sapinea, Fusarium oxysporum, Aspergillus niger, Penicillium citrinum and Penicillium italicum through the mycelial growth rate method and the Oxford cup method. The results showed that the Co(diniconazole)_2Cl_2 was with more broad-spectrum, and had antibacterial activity against the above fungi, bacteria and yeasts, especially against the pathogenic fungi;when the concentration was 2.50 mg/mL, the inhibition rate against S. sapinea reached 95.81%, and at this time, the mycostatic zone of P. italicum reached 31.77 mm in diameter. The Ag(diniconazole)_2Cl_2 was more targeted, and had the strongest inhibitory effect on S. sapinea and P. italicum; it could completely inhibit the mycelium growth of S. sapinea when the concentration was 0.50 mg/mL, and at this time, the mycostatic zone of P. italicum reached 25.07 mm in diameter, and the inhibitory effect was significantly stronger than that of A. niger and P. citrinum. So, the Co(diniconazole)_2Cl_2 and Ag(diniconazole)_2Cl_2 had good antibacterial effects on pathogenic fungi with high application potentials.

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