Position: Home > Articles > Determination for Trace Iron in Milk by Dual-indicator and Dual-wavelength Catalytic Kinetic Spectrophotometry
Hubei Agricultural Sciences
2016,55
(7)
1819-1823
双波长双指示剂催化光度法测定牛奶中的痕量铁
作 者：
王伟华;张坤
单 位：
塔里木大学生命科学学院/新疆生产建设兵团南疆特色农产品深加工重点实验室
关键词：
痕量铁;双波长;双指示剂;催化光度法;牛奶
摘 要：
以酸性介质中痕量铁(III)催化的双氧水氧化中性红和亚甲基蓝褪色原理为基础,建立了双波长双指示剂催化动力学光度法测定痕量铁的新方法。确定的最佳试验条件为HCl用量为0.5 m L,亚甲基蓝用量为3 m L,中性红用量为0.4 m L,3%的双氧水用量为0.5 m L,反应时间为8 min,反应温度为100℃,线性范围为0.4~20.0μg/L,回收率在85%~89%之间,将该方法与经典原子吸收光度法相比,具有反应时间短、准确度高、精确度好的优点,适合牛奶中痕量铁的检测。
译 名：
Determination for Trace Iron in Milk by Dual-indicator and Dual-wavelength Catalytic Kinetic Spectrophotometry
作 者：
WANG Wei-hua;ZHANG Kun;College of Life Science, Tarim University/Xinjiang Production & Construction Corps Key Laboratory of Deep Processing of Agricultural Products in South Xinjiang;
关键词：
trace iron;;dual-wavelength;;dual-indicator;;catalytic spectrophotometry;;milk
摘 要：
A new determination method for trace iron in milk by dual-wavelength and dual-indicator catalytic kinetic spectrophotometry was established based on decoloration of hydrogen peroxide oxidation neutral red and methyl thionine chloride catalyzed by the trace iron(Ⅲ) in acid medium. The optimum conditions of demonstration experiments showed as follow : the dosage of HCl, methyl thionine chloride, neutral red, and hydrogen peroxide in 3% volume fraction were 0.5 m L, 3 m L, 0.4 m L,and 0.5 m L respectively, with reaction time of 8 mins and reaction temperature of 100 ℃. The linear range was from 0.4 to20.0 μg / L while the recovery rate was in 85%~89%. Compared with the classical method atomic absorption spectroscopy, the method have advantages of less reaction time, higher accuracy and better precision, which was suitable for determining trace iron in milk.
相似文章
-
析相微萃取-分光光度法测定农产品中痕量铁 [刘国霞, 尹继颖, 赵翠翠, 张长花, 王彩红] 湖北农业科学 2011,50 (17) 3622-3624
