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Position: Home > Articles > Detection of Hsp70 Gene Expression in Heat Stressed Mice Blastocysts by RT-PCR Chinese Journal of Animal Science 2007,43 (11) 8-10

热应激处理鼠囊胚Hsp70基因表达的RT-PCR检测

作  者:
姜同泉;任登良;姜忠玲;田文儒;朱绍辉
单  位:
莱阳农学院动物科技学院
关键词:
热应激;Hsp70基因;RT-PCR;小鼠囊胚
摘  要:
将体外培养的小鼠囊胚分别施以40℃1、2、3 h和38℃1、2、3 h热应激处理,然后在37℃条件下分别恢复3、2、1 h后,提取胚胎细胞总RNA,用RT-PCR方法检测小鼠囊胚Hsp70基因的表达,以明确小鼠囊胚经热应激处理后是否有诱导型Hsp70基因表达。结果表明:各试验组,包括对照组鼠的囊胚都有诱导型Hsp70基因的表达。说明小鼠囊胚对热应激处理敏感,轻微热应激就可使诱导型Hsp70基因表达。
译  名:
Detection of Hsp70 Gene Expression in Heat Stressed Mice Blastocysts by RT-PCR
作  者:
JIANG Tong-quan,REN Deng-liang,JIANG Zhong-ling,TIAN Wen-ru,ZHU Shao-hui(Department of Animal Science and Veterinary Medicine,Laiyang Agricultural College, Shandong Qingdao,266109,China)
关键词:
heat stress;Hsp70 gene;RT-PCR;mice blastocysts
摘  要:
The mice blastocysts cultured in vitro were heat treated at 40 ℃ and 38 ℃ for 1,2,and 3 h,respectively,and then were recovered at 37 ℃ for 3,2 and 1 h,respectively,in order to detect the expression of Hsp70 gene in heat stress mice blastocysts.The total RNA in the embryonic cells was extracted and the expression of Hsp70 gene was detected by RT-PCR.The results indicated that each group of blastocysts,including the control group,had the expression of Hsp70 gene.And the above results indicated that the mice blastocysts are sensitivity to heat stress,therefore,a slight heat stress could induce Hsp70 gene expression.

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