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当前位置: 首页 > 文章 > 猪场胸膜肺炎放线杆菌分离鉴定、血清型鉴定及药敏试验 黑龙江畜牧兽医 2017 (22) 108-111+297
Position: Home > Articles > Isolation and identification,serotyping and drug sensitive test of porcine Actinobacillus pleuropneumoniae Heilongjiang Animal Science and Veterinary Medicine 2017 (22) 108-111+297

猪场胸膜肺炎放线杆菌分离鉴定、血清型鉴定及药敏试验

作  者：
袁芳艳;刘泽文;周丹娜;刘威;杨克礼;段正赢;郭锐;贝为成;阮征;田永祥
单  位：
华中农业大学
关键词：
猪传染性胸膜肺炎;胸膜肺炎放线杆菌;分离鉴定;血清型鉴定;药敏试验
摘  要：
为了确定湖北某养猪场育肥猪急性死亡的原因,采用细菌分离培养与镜检、PCR鉴定与分型等方法,并进一步对分离菌株进行了药物敏感性分析和致病性研究,利用病理组织切片技术观察病变。结果表明:分离菌株为猪胸膜肺炎放线杆菌血清5型,对昆明小鼠具有较强的致病性,能导致小鼠呼吸困难和死亡;药物敏感性分析显示病原菌对头孢噻肟、头孢噻呋、头孢拉定、头孢曲松、氟苯尼考等药物高度敏感。
译  名：
Isolation and identification,serotyping and drug sensitive test of porcine Actinobacillus pleuropneumoniae
作  者：
YUAN Fangyan;LIU Zewen;ZHOU Danna;LIU Wei;YANG Keli;DUAN Zhengying;GUO Rui;BEI Weicheng;RUAN Zheng;TIAN Yongxiang;Institute of Animal Husbandry and Veterinary/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture)/Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Hubei Academy of Agricultural Sciences;College of Veterinary Medicine,Huazhong Agricultural University;Wuhan Centers for Animal Disease Prevention and Control;
单  位：
Ministry of Agriculture
关键词：
porcine contagious pleuropneumonia;;Actinobacillus pleuropneumoniae;;isolation and identification;;serotyping identification;;drug sensitive test
摘  要：
To determine the cause of acute death in fattening swines at an industrial pig farm in Hubei Province,the pathogen was identified by isolating and culture,microscopic examination,PCR assay and bacterium classification methods. Furthermore,the drug sensitive test and pathogenicity test were carried out on isolated bacterium. Results indicated that the pathogenic bacteria was APP serovar 5,which has strong pathogenicity to Kunming mice and can cause dyspnea and death in mice. Drug sensitivity analysis showed that the pathogen is highly sensitive to cefotaxime,ceftiofur,cephradine,cefatriaxone,florfenicol and other drugs..

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