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Position: Home > Articles > Study on Autolysis Conditions of Candida lipolytica China Animal Husbandry & Veterinary Medicine 2006,33 (8) 28-30

解脂假丝酵母自溶条件的研究

作  者:
沈艳丽;扎西才旦
单  位:
青海畜牧兽医总站
关键词:
自溶法;解脂假丝酵母;神经酰胺;薄层色谱法;高效液相色谱-蒸发光散射器
摘  要:
采用细胞自溶法对影响解脂假丝酵母33M(cand id a lip oly tica)细胞中神经酰胺含量的条件进行了研究。分别应用薄层色谱(TLC)方法和高效液相色谱-蒸发光散射器(HPLC-ELSD)对其进行定性和定量分析,结果表明:解脂假丝酵母的最佳自溶条件为:自溶时间24 h,pH为8.0,自溶温度为50℃。
译  名:
Study on Autolysis Conditions of Candida lipolytica
作  者:
SHEN Yan-li(General Station of Animal Husbandry and Veterinary Medicine of Qinghai,Xining 810001,China)
关键词:
autolysis;candida lipolytica;ceramids;TLC;HPLC-ELSD
摘  要:
Effect on Ceramids of Candida lipolytica by autolysis method were studied.TLC and HPLC-ELSD were applied for quantitative and qualitative analysis respectively.The result showed that the following optimal conditions for autolysis of Candida lipolytica: autolysis time: 24 h;pH 8.0;temperature: 50℃.

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