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当前位置: 首页 > 文章 > 施用钝化剂对镉污染稻田土壤微生物学特征的影响 农业现代化研究 2012,33 (2) 234-238
Position: Home > Articles > Effects of Amendments on Soil Microbial Properties in Cadmium Contaminated Paddy Soil Research of Agricultural Modernization 2012,33 (2) 234-238

施用钝化剂对镉污染稻田土壤微生物学特征的影响

作  者：
周斌;黄道友;朱奇宏;饶中秀;刘守龙
单  位：
中国科学院亚热带农业生态研究所
关键词：
钝化剂;T-RFLP;土壤微生物多样性;土壤酶活性;镉污染稻田
摘  要：
以镉污染稻田土壤为研究对象,以土壤的微生物生物量、主要酶活性及土壤真菌和细菌的种群结构为指标,采用熏蒸提取法、常规酶学分析和末端限制性酶切片段长度多态性分析(T-RFLP)等方法,研究石灰(L)、钙镁磷肥(P)、海泡石(S)和腐植酸矿粉(H)4种钝化剂储备性施用对土壤微生物的影响。结果表明,镉污染稻田储备性施用钝化剂对土壤的微生物生物量碳(Cmi)c及磷酸酶和脲酶的活性均无显著影响,但施用石灰和海泡石可使土壤微生物生物量氮(Nmi)c分别降低33.0%和33.8%,施用钙镁磷肥使Nmic增加44.9%;施用钝化剂对土壤酶活性的影响有限,仅海泡石处理的过氧化氢酶活性显著提高(增幅达19.0%)。T-RFLP的结果表明,施用海泡石和腐植酸矿粉可使土壤真菌多样性显著降低,且4种钝化剂处理均可不同程度的导致原有T-RF片段缺失和新的T-RF片段产生,表明施用钝化剂在镉污染土壤修复过程中使土壤微生物的种群结构发生变化。
译  名：
Effects of Amendments on Soil Microbial Properties in Cadmium Contaminated Paddy Soil
作  者：
ZHOU Bin 1,2,HUANG Dao-you1,ZHU Qi-hong1,RAO Zhong-xiu1,2,LIU Shou-long1(1.Key Laboratory of Agro-ecological Processes Subtropical Region,Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha,Hunan 410125,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100039,China)
关键词：
amendment;T-RFLP;soil microbial diversity;soil enzyme activity;Cd-contaminated paddy soil
摘  要：
In purpose to study the effects of in-situ remediation of Cd-contaminated paddy soil by amendments on soil microorganisms,a field experiment that used lime(L),calcium magnesium phosphate(P),sepiolite(S) and humic acid powder(H) as amendments was conducted.And we determined soil microbial biomass(fumigation extraction method),enzyme activity(conventional methods of enzymatic analysis) and soil fungi and bacteria community structure(terminal restriction fragment length polymorphism analysis,T-RFLP).The results indicated that no significant effect of amendments on soil microbial biomass,alkaline phosphatase and urease activities was observed.However,application of lime and sepiolite significantly decreased soil microbial nitrogen(Nmic) by 33.0% and 33.8%,respectively,and the application of calcium magnesium phosphate significantly increased Nmic by 44.9%.And the catalase activity was significantly increased by sepiolite(19.0%).Moreover,significant decrease of soil fungi diversity was observed after sepiolite and humic acid powder applied,and the community structure of bacteria and fungi were changed by the application of amendments.In short,using amendments to remediate Cd-contaminated paddy soil may cause soil microbial ecological risk.

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